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The Role of Transient Receptor Potential Melastatin 7 (TRPM7) in Cell Viability: A Potential Target to Suppress

Hengrui Liu1, James P Dilger1, Jun Lin1

  • 1Department of Anesthesiology, Health Science Center, Stony Brook University, Stony Brook, NY 11794, USA.

Cancers
|January 18, 2020
PubMed

Insights

Transient Receptor Potential Melastatin 7 (TRPM7) inhibitors suppressed breast cancer cell viability by disrupting the cell cycle. TRPM7 is a potential therapeutic target for breast cancer treatment.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • The role of the divalent cation-selective channel Transient Receptor Potential Melastatin 7 (TRPM7) in breast cancer viability is not well understood.
  • Previous studies suggest TRPM7 influences the proliferation of various cancer cells.

Purpose of the Study:

  • To investigate the function of TRPM7 in breast cancer cell viability.
  • To determine if TRPM7 is a viable therapeutic target for breast cancer.

Main Methods:

  • Utilized TRPM7 inhibitors (2-aminoethyl diphenylborinate, ginsenoside Rd, waixenicin A) on TRPM7-expressing and wildtype HEK293 cells.
  • Assessed TRPM7 channel function via whole-cell currents and divalent cation influx.
  • Examined the effect of TRPM7 inhibition on breast cancer cell lines (MDA-MB-231, AU565, T47D) and a TRPM7 knock-out cell line (KO-231).
  • Analyzed cell cycle progression and cell death.

Main Results:

  • TRPM7 inhibitors preferentially suppressed the viability of TRPM7-overexpressing cells compared to wildtype cells.
  • 2-aminoethyl diphenylborinate inhibited TRPM7 channel function and breast cancer cell viability by arresting the cell cycle at the S phase, not by inducing cell death.
  • TRPM7 knock-out cells exhibited reduced divalent cation influx and proliferation.
  • TRPM7 inhibitors demonstrated preferential suppression of wildtype breast cancer cells over knock-out cells, targeting the cell cycle.

Conclusions:

  • TRPM7 plays a significant role in regulating the cell cycle of breast cancer cells.
  • TRPM7 is a promising therapeutic target for breast cancer treatment.
Keywords:
2-aminoethyl diphenylborinatebreast cancercell cyclehek293trpm7

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