Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Educational Inequalities in Well-Being in Later Life in Germany: The Role of Health Behaviours and Health Literacy.

medRxiv : the preprint server for health sciences·2026
Same author

Autologous tumor-immune effusion cocultures enable ex vivo functional profiling of radiotherapy-immunotherapy combinations.

Journal of experimental & clinical cancer research : CR·2026
Same author

Differential immune infiltrates in histomorphologic Wilms tumor regions identify prognostic macrophages.

Molecular therapy. Oncology·2026
Same author

Spontaneous transanal evisceration of the small bowel through perforation of the sigmoid colon: a case report and new surgical approach.

International journal of surgery case reports·2026
Same author

FASN targeting by G28UCM impairs mitochondrial fatty acid synthesis and reveals a FASN-SDHB synthetic interaction.

Pharmacological research·2026
Same author

The impact of national context on COVID-19 vaccine hesitancy across Europe.

BMC public health·2026

Related Experiment Video

Updated: Dec 30, 2025

Preparation of Light-responsive Membranes by a Combined Surface Grafting and Postmodification Process
12:00

Preparation of Light-responsive Membranes by a Combined Surface Grafting and Postmodification Process

Published on: March 21, 2014

12.2K

Enhanced Protein Immobilization on Polymers-A Plasma Surface Activation Study.

Felicia Wieland1, Richard Bruch1,2, Michael Bergmann1

  • 1Laboratory for Sensors, Department of Microsystems Engineering, University of Freiburg, 79110 Freiburg, Germany.

Polymers
|January 18, 2020
PubMed
Summary

Plasma treatment effectively activates polymer surfaces for enhanced biomolecule immobilization in biosensors. Optimal conditions involve a 5-minute treatment with 50% oxygen/nitrogen at 1000W and 80°C, ensuring homogenous distribution.

Keywords:
biosensorsoxygen plasmaprotein immobilizationsurface activation

More Related Videos

Wet Chemistry and Peptide Immobilization on Polytetrafluoroethylene for Improved Cell-adhesion
06:15

Wet Chemistry and Peptide Immobilization on Polytetrafluoroethylene for Improved Cell-adhesion

Published on: August 15, 2016

8.1K
Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
11:13

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

Published on: August 20, 2018

11.5K

Related Experiment Videos

Last Updated: Dec 30, 2025

Preparation of Light-responsive Membranes by a Combined Surface Grafting and Postmodification Process
12:00

Preparation of Light-responsive Membranes by a Combined Surface Grafting and Postmodification Process

Published on: March 21, 2014

12.2K
Wet Chemistry and Peptide Immobilization on Polytetrafluoroethylene for Improved Cell-adhesion
06:15

Wet Chemistry and Peptide Immobilization on Polytetrafluoroethylene for Improved Cell-adhesion

Published on: August 15, 2016

8.1K
Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
11:13

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

Published on: August 20, 2018

11.5K

Area of Science:

  • Materials Science
  • Surface Chemistry
  • Biotechnology

Background:

  • Polymers are increasingly used as low-cost, versatile substrates for biosensor development.
  • Effective biomolecule immobilization is crucial for biosensor performance.
  • Surface activation techniques are necessary to enhance biomolecule attachment to polymer surfaces.

Purpose of the Study:

  • To systematically investigate and optimize plasma treatment parameters for polymer surface activation.
  • To determine the optimal conditions for maximizing biomolecule immobilization efficiency and homogeneity on various polymer substrates.
  • To evaluate the effectiveness of plasma treatment using surface characterization techniques.

Main Methods:

  • Systematic optimization of plasma treatment parameters: power, time, substrate temperature, and gas composition (oxygen/nitrogen).
  • Application of plasma treatment to various polymeric surfaces.
  • Surface characterization using Scanning Electron Microscopy (SEM), X-ray Photoelectron Spectroscopy (XPS), and contact angle measurements.
  • Assessment of biomolecule immobilization efficiency and distribution.

Main Results:

  • Identified optimal plasma treatment conditions: 5 minutes, 1000 W power, 80 °C substrate temperature, and 50% oxygen/nitrogen gas mixture.
  • Achieved the highest biomolecule immobilization efficiency and homogenous distribution under these optimized conditions.
  • Confirmed surface modifications and functional group generation via SEM, XPS, and contact angle analysis.

Conclusions:

  • Plasma treatment is a highly effective method for activating polymer surfaces for biosensing applications.
  • Optimized plasma parameters significantly enhance biomolecule immobilization, leading to improved biosensor performance.
  • The study provides a comprehensive guideline for utilizing plasma technology in polymer-based biosensor fabrication.