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Related Experiment Video

Updated: Dec 30, 2025

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
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Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

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TRPC Channels in the SOCE Scenario.

Jose J Lopez1, Isaac Jardin1, Jose Sanchez-Collado1

  • 1Department of Physiology (Cell Physiology Research Group), Institute of Molecular Pathology Biomarkers, University of Extremadura, 10003-Caceres, Spain.

Cells
|January 18, 2020
PubMed
Summary

Transient receptor potential (TRP) channels, particularly TRPC1, are involved in store-operated calcium (SOC) entry. This review explores their function alongside STIM1 and Orai1 in regulating calcium influx.

Keywords:
Orai1STIM1TRPC1calcium influxstore-operated Ca2+ entry (SOCE)

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Area of Science:

  • Cellular Physiology
  • Ion Channel Biology
  • Molecular Mechanisms of Calcium Signaling

Background:

  • Transient receptor potential (TRP) proteins are non-selective cation channels crucial for physiological functions.
  • Canonical TRP (TRPC) channels have been implicated in store-operated calcium (SOC) channels, but their exact role remains debated.
  • STIM1 and Orai1 are established components of CRAC (calcium release-activated calcium) channels.

Purpose of the Study:

  • To review the functional role of TRPC channels within the STIM1-Orai1 pathway.
  • To elucidate the interplay between TRPC proteins, STIM1, and Orai1 in calcium influx.
  • To clarify the contribution of TRPC channels to store-operated calcium entry.

Main Methods:

  • Literature review of studies investigating TRPC channel function.
  • Analysis of evidence for STIM1-mediated gating of TRPC1 and Orai1.
  • Examination of the molecular interactions between STIM1, Orai1, and TRPC1.

Main Results:

  • STIM1 gates both Orai1 and TRPC1 to mediate non-selective cation currents (ISOC).
  • TRPC1's involvement in store-operated calcium entry necessitates both STIM1 and Orai1.
  • TRPC1 and other TRPC proteins may modulate Orai1 channel activity.

Conclusions:

  • TRPC channels, especially TRPC1, play a significant role in the STIM1-Orai1 mediated store-operated calcium entry.
  • The precise mechanisms by which TRPC proteins influence Orai1 function warrant further investigation.
  • Understanding TRPC channel involvement in calcium signaling is vital for comprehending various physiological processes.