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Detecting and analyzing murine innate lymphoid cells.

Alejandra Gomez-Cadena1, Pedro Romero2, Sara Trabanelli1

  • 1Department of Oncology UNIL CHUV, University of Lausanne, Lausanne, Switzerland; Ludwig Institute for Cancer Research, University of Lausanne, Lausanne, Switzerland.

Methods in Enzymology
|January 18, 2020
PubMed
Summary

Innate lymphoid cells (ILCs) are crucial for immune homeostasis and tissue repair. This chapter details methods for identifying and isolating diverse ILC subsets in mice using multiparametric flow cytometry.

Keywords:
Immune regulationInnate immunityInnate lymphoid cells (ILC)

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Innate lymphoid cells (ILCs) are vital immune cells involved in maintaining tissue homeostasis and regulating immune responses.
  • Recent advancements have identified distinct ILC subsets in various murine organs.
  • ILCs are primarily tissue-resident but can expand locally during physiological or pathological conditions.

Purpose of the Study:

  • To describe methods for identifying and isolating different subsets of Innate lymphoid cells (ILCs) in murine models.
  • To outline the functional analysis of isolated ILC subsets.

Main Methods:

  • Multiparametric flow cytometry is presented as the most efficient method for ILC analysis.
  • Detailed protocols for identifying ILCs based on cell-surface and intracellular markers are described.
  • Techniques for isolating specific ILC subsets from lymphoid and non-lymphoid organs are explained.

Main Results:

  • The chapter provides a comprehensive guide to ILC identification and isolation in mice.
  • It highlights the importance of multiparametric flow cytometry for distinguishing ILC subsets.
  • Functional analysis methods for these subsets are also discussed.

Conclusions:

  • Accurate identification and isolation of ILC subsets are crucial for understanding their roles in immunity.
  • Multiparametric flow cytometry is an essential tool for ILC research in murine models.
  • This chapter serves as a practical guide for researchers studying ILCs.