Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

5.1K
The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
5.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Plant-sourced cooking oil consumption is associated with lower total mortality in a longitudinal nationwide cohort study.

Clinical nutrition (Edinburgh, Scotland)·2020
Same author

LINC01116 promotes tumor proliferation and neutrophil recruitment via DDX5-mediated regulation of IL-1β in glioma cell.

Cell death & disease·2020
Same author

Metal-Organic Framework Membrane Nanopores as Biomimetic Photoresponsive Ion Channels and Photodriven Ion Pumps.

Angewandte Chemie (International ed. in English)·2020
Same author

Graphdiyne oxide: a new carbon nanozyme.

Chemical communications (Cambridge, England)·2020
Same author

Egg and egg-sourced cholesterol consumption in relation to mortality: Findings from population-based nationwide cohort.

Clinical nutrition (Edinburgh, Scotland)·2020
Same author

A blood-based 22-gene expression signature for hepatocellular carcinoma identification.

Annals of translational medicine·2020

Related Experiment Video

Updated: Dec 30, 2025

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function
09:20

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function

Published on: May 4, 2021

4.1K

MiR-30a-5p accelerates adipogenesis by negatively regulating Sirtuin 1.

Shanshan Cui1, Chaitanya Brijmohan Soni2, Juan Xie3

  • 1China Agriculture University Beijing, China.

International Journal of Clinical and Experimental Pathology
|January 18, 2020
PubMed
Summary

MicroRNA-30a-5p promotes adipocyte differentiation by targeting Sirtuin 1 (SIRT1). This finding suggests miR-30a-5p as a potential therapeutic target for obesity.

Keywords:
ObesitySirtuin 1adipocyte differentiationmiR-30a-5p

More Related Videos

Investigation of Beige Fat Biology and Metabolism Using the CRISPR SunTag-p65-HSF1 Activation System
09:52

Investigation of Beige Fat Biology and Metabolism Using the CRISPR SunTag-p65-HSF1 Activation System

Published on: January 6, 2023

2.7K
Encapsulation Thermogenic Preadipocytes for Transplantation into Adipose Tissue Depots
08:30

Encapsulation Thermogenic Preadipocytes for Transplantation into Adipose Tissue Depots

Published on: June 2, 2015

9.7K

Related Experiment Videos

Last Updated: Dec 30, 2025

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function
09:20

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function

Published on: May 4, 2021

4.1K
Investigation of Beige Fat Biology and Metabolism Using the CRISPR SunTag-p65-HSF1 Activation System
09:52

Investigation of Beige Fat Biology and Metabolism Using the CRISPR SunTag-p65-HSF1 Activation System

Published on: January 6, 2023

2.7K
Encapsulation Thermogenic Preadipocytes for Transplantation into Adipose Tissue Depots
08:30

Encapsulation Thermogenic Preadipocytes for Transplantation into Adipose Tissue Depots

Published on: June 2, 2015

9.7K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Obesity is a chronic metabolic disease driven by excess fat accumulation.
  • Disordered preadipocyte differentiation is a key driver of adipogenesis.
  • Understanding adipocyte differentiation mechanisms is crucial for metabolic disease research.

Purpose of the Study:

  • To investigate the role of microRNA-30a-5p (miR-30a-5p) in adipocyte differentiation.
  • To elucidate the molecular targets and mechanisms underlying miR-30a-5p's function in adipogenesis.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR) for miR-30a and Sirtuin 1 (SIRT1) mRNA.
  • Western blot (WB) for SIRT1, PPARγ, C/EBPα, and FABP4 protein levels.
  • Luciferase reporter assay to confirm miR-30a-5p and SIRT1 interaction.

Main Results:

  • miR-30a-5p expression increased during 3T3-L1 cell differentiation.
  • Overexpression of miR-30a-5p enhanced adipocyte differentiation markers (PPARγ, C/EBPα, FABP4, triglyceride) and reduced cell proliferation.
  • SIRT1 was identified as a direct target of miR-30a-5p; SIRT1 supplementation inhibited differentiation.

Conclusions:

  • miR-30a-5p regulates 3T3-L1 cell differentiation by targeting SIRT1.
  • miR-30a-5p emerges as a potential novel therapeutic target for obesity treatment.