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Updated: Dec 30, 2025
Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
Published on: November 30, 2016
MicroRNA-142-5p facilitates the pathogenesis of ulcerative colitis by regulating SOCS1
Jing Han1,2, Yawei Li3, Hong Zhang1
1Department of Gastroenterology, The East Branch of The Second Hospital of Hebei Medical University, Hebei Key Laboratory of Gastroenterology, Hebei Institute of Gastroenterology Shijiazhuang, China.
Background:
Increasing evidence suggests that abnormal levels of microRNAs (miRNAs) are associated with ulcerative colitis (UC). It has been demonstrated that microRNA (miR)-142-5p was upregulated in UC patients. However, it remains unclear what the role of miR-142-5p is in UC.
Methods:
Samples from patients with active UC and healthy controls were performed with miRNA microarray to identify miRNAs involved in the pathogenesis of UC. The results of quantitative RT-PCR verified that miR-142-5p was upregulated in UC patients. Meanwhile, the decreased expression of suppressor of cytokine signaling 1 (SOCS1) was also detected at mRNA and protein levels. The regulatory effect of miR-142-5p on SOCS1 was evaluated by luciferase reporter assay. Levels of IL-6 or IL-8 were detected by quantitative RT-PCR or enzyme-linked immunosorbent assay in HT-29 cells to evaluate the roles of SOCS1 or miR-142-5p in the progression of UC.
Results:
The expression level of miR-142-5p was significantly upregulated and inversely correlated with SOCS1. Luciferase experiments showed that miR-142-5p interfered with the expression of SOCS1 by directly targeting its 3'-UTR. Furthermore, the level of miR-142-5p plays an important role in the secretion of IL-6 and IL-8. Moreover, lost function of SOCS1 reversed the miR-142-5p inhibitory effect.
Conclusions:
These results indicate that miR-142-5p improved the intestinal inflammation of active-UC patients by downregulating SOCS1 expression and increasing the cytokines IL-6 and IL-8 secretion.
Insights
MicroRNA (miR)-142-5p is upregulated in ulcerative colitis (UC) and worsens intestinal inflammation by downregulating SOCS1, increasing IL-6 and IL-8. This suggests miR-142-5p as a potential therapeutic target for UC.
Area of Science:
- Gastroenterology
- Molecular Biology
- Immunology
Background:
- Abnormal microRNA (miRNA) levels are linked to ulcerative colitis (UC).
- MicroRNA (miR)-142-5p is notably upregulated in UC patients.
- The specific role of miR-142-5p in UC pathogenesis requires elucidation.
Purpose of the Study:
- To investigate the role of miR-142-5p in the context of ulcerative colitis (UC).
- To determine the relationship between miR-142-5p and suppressor of cytokine signaling 1 (SOCS1) in UC.
- To assess the impact of miR-142-5p and SOCS1 on inflammatory cytokine production in UC.
Main Methods:
- miRNA microarray and quantitative RT-PCR were used to analyze miRNA expression in UC patients and controls.
- The regulatory interaction between miR-142-5p and SOCS1 was assessed using luciferase reporter assays.
- Levels of IL-6 and IL-8 were measured to evaluate the functional roles of miR-142-5p and SOCS1 in UC progression.
Main Results:
- miR-142-5p expression was significantly upregulated in UC patients and inversely correlated with SOCS1 expression.
- miR-142-5p directly targets the 3'-UTR of SOCS1, inhibiting its expression.
- miR-142-5p influences the secretion of IL-6 and IL-8, and SOCS1 dysfunction reverses these effects.
Conclusions:
- miR-142-5p exacerbates intestinal inflammation in active UC by downregulating SOCS1.
- This downregulation leads to increased secretion of pro-inflammatory cytokines IL-6 and IL-8.
- Targeting miR-142-5p may offer a therapeutic strategy for managing ulcerative colitis.
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