Related Experiment Video
Updated: Dec 30, 2025
10:37
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Published on: November 15, 2017
12.5K
Beyond mass spectrometry, the next step in proteomics
1Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.
Science Advances
|January 18, 2020
Summary
New protein sequencing methods offer superior sensitivity and scalability compared to current mass spectrometry (MS) techniques. These advancements promise to advance proteomics and its applications in medicine.
Area of Science:
- Biochemistry
- Molecular Biology
- Proteomics
Background:
- Proteins play critical roles in disease development and treatment.
- Early protein sequencing (1951) pioneered biopolymer analysis.
- Genomics and transcriptomics have advanced rapidly, but proteomics lags.
- Current proteomics relies on mass spectrometry (MS), which classifies proteins and requires high abundance (billions of copies).
Purpose of the Study:
- To survey alternative protein identification and sequencing technologies.
- To highlight methods with potential to surpass mass spectrometry (MS).
Main Methods:
- Review of emerging proteomics technologies.
- Comparative analysis of sensitivity, scalability, and bioinformatics adaptability.
Main Results:
- Several alternative technologies demonstrate superior sensitivity to MS.
- These methods show promise for scalability.
- Adaptability to bioinformatics for amino acid sequence determination is feasible.
Conclusions:
- Emerging proteomics techniques offer significant advantages over current MS-based methods.
- These alternatives could revolutionize protein identification and sequencing.
- Advancements in proteomics will drive progress in medicine and disease research.
Related Concept Videos
01:33Peptide Identification Using Tandem Mass Spectrometry
8.0K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.0K
01:33Proteomics
9.2K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.2K
01:21Tandem Mass Spectrometry
2.2K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.2K
01:19MALDI-TOF Mass Spectrometry
6.4K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
6.4K