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[The Diagnostic Value of TSPOT.TB in Children with Tuberculosis]

Gui-Xiang Jian1, Yan-Feng Huang1,2,3,4,5

  • 1Department of Infectious Diseases, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.

Sichuan Da Xue Xue Bao. Yi Xue Ban = Journal of Sichuan University. Medical Science Edition
|January 18, 2020
PubMed

Insights

The T-cell enzyme linked immunospot (TSPOT.TB) test shows high diagnostic value for pediatric tuberculosis. This test offers excellent sensitivity and specificity, aiding in the rapid diagnosis of tuberculosis in children.

Area of Science:

  • Pediatric Infectious Diseases
  • Immunodiagnostics
  • Tuberculosis Research

Background:

  • Tuberculosis (TB) remains a significant global health challenge, particularly in children.
  • Accurate and rapid diagnostic tools are crucial for effective TB management in pediatric populations.
  • The T-cell enzyme linked immunospot (TSPOT.TB) assay is a promising interferon-gamma release assay (IGRA) for TB diagnosis.

Purpose of the Study:

  • To evaluate the diagnostic accuracy of the TSPOT.TB assay in children suspected of having tuberculosis.
  • To determine the sensitivity, specificity, and predictive values of TSPOT.TB for pediatric TB diagnosis.

Main Methods:

  • A retrospective analysis of clinical data from 2,348 children who underwent TSPOT.TB testing was conducted.
  • Diagnostic performance metrics including sensitivity, specificity, positive predictive value, and negative predictive value were calculated.
  • Receiver operating characteristic (ROC) curve analysis was performed to assess the overall diagnostic performance.

Main Results:

  • TSPOT.TB demonstrated high diagnostic accuracy with a sensitivity of 84.0% and specificity of 99.1%.
  • The positive predictive value was 93.1%, and the negative predictive value was 97.8%.
  • The area under the ROC curve (AUC) for A and B antigens was 0.893 and 0.883, respectively (P<0.05), with higher sensitivity observed for pulmonary TB and increasing with age.

Conclusions:

  • TSPOT.TB exhibits significant diagnostic value for pediatric tuberculosis.
  • The assay can rapidly assist in the diagnosis of TB in children, offering high reliability.
Abstract

Keywords:
ChildrenROC curveTSPOT.TBTuberculosis

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