A 4,5-quinolimide-based fluorescent sensor for sequential detection of Cu²⁺ and cysteine in water and living cells...

In this study, a new 4,5-quinolimide-based fluorescent sensor BNC was synthesized and characterized. BNC showed single selectivity for Cu²⁺via the "turn-off" fluorescence among various common metal ions. After forming a 1:1 stoichiometric complex with Cu²⁺, the detection limit (LOD) of BNC for Cu²⁺ was measured to be 0.44 μM. Subsequently, the in situ generated BNC-Cu²⁺ complex had been used for sensing Cys with the LOD of 1.5 μM through the displacement strategy, resulting in the revivable emission of BNC. According to the "off-on-off" fluorescence cycle of BNC generated by the alternate addition of Cu²⁺ and Cys, a reversible memorized device with "read-write-read-erase" behavior was constructed at the molecular level. Furthermore, the recoveries of Cu²⁺ in lake water with BNC were in the range of 95.0-105%. And sequential fluorescence imagings of BNC for Cu²⁺ and Cys were successfully applied in living yeast cells.