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Electrosprayed whey protein-based nanocapsules for β-carotene encapsulation.

Rui M Rodrigues1, Philippe E Ramos1, M F Cerqueira2

  • 1CEB - Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.

Food Chemistry
|January 18, 2020
PubMed
Summary

Whey protein isolate (WPI) nanocapsules were created using electrospray to encapsulate beta-carotene. Higher ethanol concentrations destabilized WPI, affecting nanocapsule size and structure, indicating potential for novel bioactive compound protection.

Keywords:
Electrohydrodynamic processEncapsulationFunctional compoundsNanotechnology

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Area of Science:

  • Food Science and Technology
  • Materials Science
  • Biotechnology

Background:

  • Whey protein isolate (WPI) is a valuable biomaterial for encapsulation.
  • Beta-carotene is a sensitive bioactive compound requiring protection.
  • Electrospray is a novel technique for nanoparticle production.

Purpose of the Study:

  • To produce beta-carotene loaded nanocapsules using WPI and electrospray.
  • To investigate the effect of ethanol concentration on nanocapsule properties.
  • To evaluate WPI nanocapsules as a delivery system for beta-carotene.

Main Methods:

  • Electrospray of WPI solutions with varying ethanol concentrations (5, 10, 15%).
  • Solubilization of beta-carotene in ethanol-WPI solutions.
  • Characterization of nanocapsule morphology, size, and molecular organization (dried and hydrated states).
  • Analysis of protein unfolding using free sulfhydryl group reactivity and fluorescence.

Main Results:

  • Dried nanocapsule sizes ranged from 227 to 283 nm.
  • Hydration significantly increased nanocapsule size, with larger sizes at higher ethanol concentrations.
  • Increased ethanol concentrations led to protein unfolding and destabilization.
  • Electrosprayed WPI nanocapsules demonstrated potential for beta-carotene encapsulation.

Conclusions:

  • Electrospray is a viable method for producing WPI-based nanocapsules for beta-carotene.
  • Ethanol concentration critically influences WPI structure and resulting nanocapsule characteristics.
  • These nanocapsules offer a promising solution for protecting sensitive bioactive compounds in industrial applications.