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Related Concept Videos

Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

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Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
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Photoluminescence: Applications01:14

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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Related Experiment Video

Updated: Dec 30, 2025

Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence
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Reversible Oxygen Sensing Based on Multi-Emission Fluorescence Quenching.

Efe Armagan1,2, Shankar Thiyagarajan1, Kongchang Wei1

  • 1Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Biomimetic Membranes and Textiles, Lerchenfeldstrasse 5, CH-9014 St. Gallen, Switzerland.

Sensors (Basel, Switzerland)
|January 19, 2020
PubMed
Summary

This study introduces novel, purely organic fluorescent sensors for oxygen detection. These cost-effective, metal-free sensors offer reversible and reproducible oxygen sensing, ideal for applications like food packaging.

Keywords:
carbon nanodotsfluorescence-based oxygen quenchingmolecular fluorophoresmulti-emissionoptical oxygen sensing

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Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
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Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Biochemistry

Background:

  • Oxygen is vital in biological processes and food spoilage.
  • Current oxygen sensors often rely on phosphorescent organometallic complexes.
  • A need exists for novel, non-destructive oxygen sensing methods.

Purpose of the Study:

  • To develop a new optical oxygen sensing method using fluorescence quenching.
  • To synthesize and characterize novel multi-emissive purely organic emitters.
  • To evaluate the performance of these emitters as oxygen sensors.

Main Methods:

  • One-pot hydrothermal synthesis of organic emitters from p-phenylenediamine (PPD) and urea.
  • Characterization of synthesized materials using ESI-QTOF and LC-MS.
  • Embedding emitters in a poly(vinyl alcohol) matrix for sensor fabrication and testing.

Main Results:

  • Successfully synthesized multi-emissive purely organic emitters.
  • Observed non-linear, reversible fluorescence quenching by oxygen (0-8 kPa range).
  • Achieved low detection limits (0.67-0.89 kPa) with high reproducibility and minimal humidity interference.

Conclusions:

  • Developed a novel, metal-free, purely organic fluorescent oxygen sensor.
  • The sensor demonstrates promising performance for oxygen detection in various applications, including food packaging.
  • Cost-effectiveness and reversibility make these sensors a viable alternative to existing technologies.