Cellulose, pectin and water in cell walls determine apple flesh viscoelastic mechanical properties

The viscoelastic mechanical properties are important quality traits for fleshy fruit uses. The contribution of cell wall polysaccharides chemistry and organization on their variability was studied in six varieties of apple. Correlation between damping and storage modulus of plasmolyzed tissue distinguished better apple varieties on their viscoelasticity than fresh samples. Galactose, arabinose and uronic acids correlated positively with the storage modulus of fresh apple samples (E'_f). These corresponded to 4-linked galactan but no specific arabinose linkage. Galacturonic acid branched on O-3 and terminal rhamnose correlated negatively with E'_f. These correlations formed two groups of fruit except for branched methyl-esterified galacturonic. Solid-state ¹³C NMR spectroscopy analyses showed that E'_f correlated negatively with cellulose C4 T_1ρ^H relaxation and positively with pectin methyl esters T_HH proton diffusion. The results point to the key roles of pectin structure and hydration and cellulose microfibrils distribution on apple mechanical properties.