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Structural Studies of Overlapping Dinucleosomes in Solution.

Atsushi Matsumoto1, Masaaki Sugiyama2, Zhenhai Li1

  • 1Institute for Quantum Life Science, National Institutes for Quantum and Radiological Science and Technology, Kizugawa, Japan.

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|January 19, 2020
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Summary

Overlapping dinucleosomes (OLDNs), structures of hexasomes and octasomes, were studied using scattering and modeling. Histone tails are crucial for stabilizing OLDN conformations and their DNA binding.

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Area of Science:

  • Molecular Biology
  • Structural Biology
  • Biophysics

Background:

  • Overlapping dinucleosomes (OLDNs) are formed by nucleosome collision, involving hexasomes and octasomes.
  • Nucleosome remodeling factors are implicated in OLDN formation.
  • Understanding OLDN structure is key to chromatin dynamics and gene regulation.

Purpose of the Study:

  • To investigate the solution structure of overlapping dinucleosomes (OLDNs).
  • To elucidate the role of histone tails in OLDN conformation and stability.
  • To provide insights into chromatin dynamics relevant to gene regulation.

Main Methods:

  • Integration of small-angle X-ray scattering (SAXS) and small-angle neutron scattering (SANS).
  • Computational modeling, including normal mode analysis.
  • Molecular dynamics simulations.

Main Results:

  • Structural models revealed OLDNs adopt diverse conformations.
  • Histone tails, absent in crystal structures, significantly improved model quality.
  • Histone tails stabilize OLDNs by binding to both hexasomal and octasomal DNA at the interface.

Conclusions:

  • Histone tails play a critical role in stabilizing overlapping dinucleosome structures.
  • The study provides models for OLDN conformational dynamics.
  • Findings contribute to understanding chromatin dynamics in gene regulation.