miR-148 targets CiGadd45ba and CiGadd45bb to modulate the inflammatory response to bacterial infection in grass carp
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Updated: Dec 30, 2025
A Mouse Model for Pathogen-induced Chronic Inflammation at Local and Systemic Sites
Published on: August 8, 2014
Yuan Fang1, Xiao-Yan Xu2, Yubang Shen2
1Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Shanghai Ocean University), Ministry of Education, Shanghai, 201306, China.
Abstract:
In grass carp (Ctenopharyngodon idella), septicemia is a systemic inflammatory response to bacterial infection. Once infected bacteria, a hyper-inflammatory state that could lead to septic shock and death. There is increasing evidence that microRNAs are involved in the regulation of the inflammatory response. Ctenopharyngodon idella growth arrest and DNA damage-inducible 45 ba and bb (CiGadd45ba and CiGadd45bb) are two subtypes of Gadd45b. In the present study, miR-148 was confirmed to be involved in the inflammatory response after infection with Aeromonas hydrophila. Dual-luciferase reporter assays and miRNA expression profiling confirmed that miR-148 targeted both CiGadd45ba and CiGadd45bb. Transfection with miR148 mimics and inhibitors altered the expression levels of proinflammatory genes, suggesting that miRNAs regulate the immune response in grass carp. Our results provide a theoretical basis for studying the molecular mechanism underlying the regulation of inflammation by miR-148 in grass carp.
Insights
MicroRNAs, specifically miR-148, play a key role in regulating the inflammatory response in grass carp (Ctenopharyngodon idella) following bacterial infection. This study identifies miR-148 targeting of CiGadd45ba and CiGadd45bb as a crucial mechanism in grass carp immunity.
Area of Science:
- * Molecular Biology
- * Immunology
- * Aquatic Animal Health
Background:
- * Septicemia in grass carp (Ctenopharyngodon idella) is a severe inflammatory condition caused by bacterial infection, potentially leading to septic shock.
- * MicroRNAs (miRNAs) are increasingly recognized for their role in modulating inflammatory responses.
- * Ctenopharyngodon idella growth arrest and DNA damage-inducible 45 ba and bb (CiGadd45ba and CiGadd45bb) are identified as key players in the inflammatory pathway.
Purpose of the Study:
- * To investigate the role of miR-148 in the inflammatory response of grass carp after Aeromonas hydrophila infection.
- * To identify the target genes of miR-148 involved in this inflammatory process.
- * To elucidate the regulatory mechanism of miR-148 in the immune response of grass carp.
Main Methods:
- * Confirmation of miR-148 involvement in the inflammatory response post-Aeromonas hydrophila infection.
- * Dual-luciferase reporter assays and miRNA expression profiling to determine miR-148 targets.
- * Transfection experiments using miR148 mimics and inhibitors to assess effects on proinflammatory gene expression.
Main Results:
- * miR-148 was confirmed to be involved in the inflammatory response in grass carp infected with Aeromonas hydrophila.
- * Dual-luciferase reporter assays and miRNA expression profiling demonstrated that miR-148 directly targets both CiGadd45ba and CiGadd45bb.
- * Modulation of miR-148 levels through mimics and inhibitors significantly altered the expression of proinflammatory genes.
Conclusions:
- * miR-148 plays a significant role in regulating the immune response and inflammation in grass carp.
- * The targeting of CiGadd45ba and CiGadd45bb by miR-148 is a key molecular mechanism in this regulation.
- * These findings provide a theoretical foundation for understanding miR-148-mediated inflammation control in grass carp.
