Related Experiment Video

Updated: Dec 30, 2025

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

13.3K

Relationship between changes in the exon-recognition machinery and SLC22A1 alternative splicing in hepatocellular

Meraris Soto1, Maria Reviejo1, Ruba Al-Abdulla1

  • 1HEVEFARM Group, University of Salamanca, IBSAL, Salamanca, Spain.

Biochimica Et Biophysica Acta. Molecular Basis of Disease
|January 19, 2020
PubMed

Insights

Aberrant splicing of SLC22A1 pre-mRNA increases in hepatocellular carcinoma (HCC), reducing functional organic cation transporter 1 (OCT1) expression. This highlights the role of splicing changes in cancer drug response.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Genetics

Background:

  • Aberrant pre-mRNA processing by the spliceosome influences cancer cell phenotypes.
  • Hepatocellular carcinoma (HCC) exhibits impaired response to sorafenib due to reduced organic cation transporter 1 (OCT1) expression, linked to aberrant splicing of its gene (SLC22A1).

Purpose of the Study:

  • To investigate the relationship between alternative splicing of SLC22A1 pre-mRNA and the expression of exon-recognition machinery genes in HCC.
  • To validate findings using paired tumor and non-tumor tissues.

Main Methods:

  • In silico analysis of public databases.
  • Taqman Low-Density Arrays for validation in 25 resected HCC tumors.
  • Analysis of paired tumor and adjacent non-tumor tissues.

Main Results:

  • Significant alternative splicing of SLC22A1 was observed in non-tumor tissue, further increased in HCC tumors irrespective of stage.
  • Splicing perturbations correlated with altered expression of proteins involved in exon recognition.
  • Paired sample analysis confirmed aberrant splicing's role in functional OCT1 expression reduction in HCC.

Conclusions:

  • Aberrant splicing of SLC22A1 is a key factor in reduced OCT1 expression in HCC.
  • Changes in the exon-recognition machinery may impact other protein expressions in HCC.
  • Further research into spliceosome-associated genes and aberrant splicing in HCC is warranted.
Keywords:
CancerChemoresistanceChemotherapyLiverOCT1Spliceosome

More Related Videos

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.3K
Using the E1A Minigene Tool to Study mRNA Splicing Changes10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

5.3K

Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
24.5K
Alternative RNA Splicing02:18

Alternative RNA Splicing

4.7K
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.1K
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon...
4.0K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
8.0K
Leaky Scanning02:28

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.6K