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Expression Analysis, Functional Marker Development and Verification of AgFNSI in Celery
Jun Yan1, Lizhong He1, Shuang Xu1
1Horticulture Research Institute, Shanghai Academy of Agricultural Sciences; Key Laboratory of Protected Horticulture Technology, Shanghai, 201403, China.
Scientific Reports
|January 19, 2020
Summary
Celery
Area of Science:
- Plant biochemistry and genetics.
- Focus on flavonoid biosynthesis in celery.
Background:
- Apigenin, a valuable medicinal flavonoid, is synthesized in celery.
- Flavone synthase I (FNSI) catalyzes the final step in apigenin biosynthesis.
Purpose of the Study:
- To investigate the promoter region of the celery AgFNSI gene.
- To identify genetic variations associated with apigenin content in celery.
Main Methods:
- Obtained and analyzed the 1492 bp promoter sequence of celery AgFNSI.
- Performed comparative genomic and promoter sequence analysis across different celery accessions.
- Designed and validated functional markers based on SNPs in AgFNSI alleles.
Main Results:
- The AgFNSI promoter contains responsive elements for methyl jasmonate (MeJA), light, and anaerobic conditions, plus MYB binding sites.
- AgFNSI expression is influenced by temperature, UV-B, water deficit, and MeJA.
- Identified four allelic variations in AgFNSI, with AgFNSIa linked to high apigenin content and AgFNSIc to low content.
- Developed and validated functional marker AFPA1/AFPB1, which significantly correlates with apigenin levels in celery.
Conclusions:
- The functional marker AFPA1/AFPB1 is a reliable indicator of apigenin content in celery.
- This marker can be utilized for the genetic improvement of apigenin accumulation in celery cultivars.
