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Updated: Dec 30, 2025

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

Complementary Fluorescence Emission and Second Harmonic Spectra Improve Bilayer Characterization.

Radha Ranganathan¹, Asher J Burkin², Miroslav Peric²

¹Department of Physics, California State University Northridge, Northridge, CA, 91330, USA. radha.ranganathan@csun.edu.

Journal of Fluorescence

|January 19, 2020

Summary

This summary is machine-generated.

Laurdan fluorescence and second harmonic (SH) spectra reveal distinct emission patterns in solvents and phospholipid bilayers. These findings offer new insights into lipid packing and domain evolution, even in the gel phase.

Keywords:

Fluorescence Fluorescence lineshape Laurdan Phospholipid bilayers

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Area of Science:

Molecular spectroscopy
Biophysics
Materials science

Background:

Laurdan is a fluorescent probe used to study lipid bilayers.
Understanding lipid packing and domain formation is crucial for membrane function.

Purpose of the Study:

To investigate Laurdan fluorescence emission and second harmonic (SH) spectra in various solvents and phospholipid bilayers.
To elucidate the relationship between Laurdan conformation, lipid packing, and domain evolution.

Main Methods:

Complementary analysis of Laurdan fluorescence and SH spectra.
Computation of SH spectra using electro spin resonance spectroscopy methods.
Fitting spectra to log-normal distributions.
Temperature-dependent measurements.

Main Results:

SH spectra revealed two emissions in nonpolar/protic solvents and bilayer phases, and a single line in aprotic polar solvents.
A previously undetected 432 nm blue line was observed in the gel phase.
Temperature trajectories indicated inhomogeneous lipid packing and domain evolution.
The blue line corresponds to Laurdan in tightly packed regions, while the red line relates to Laurdan-water coupled states.

Conclusions:

Laurdan conformation (planar/nonplanar) correlates with emission wavelengths.
SH spectroscopy enhances the detection of subtle spectral features.
Temperature-dependent spectral changes provide insights into lipid domain dynamics and membrane curvature preferences.