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miR-190b promotes tumor growth and metastasis via suppressing NLRC3 in bladder carcinoma
Zhaohui Chen1, Likun Yang1, Liang Chen1
1Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Abstract:
Bladder cancer is one of the most common urogenital malignancies. However, its pathogenesis, especially molecular mechanisms remain elusive. Thus, understanding the molecular mechanisms underlying bladder cancer is important for the discovery of novel therapeutic paradigms for these diseases. In current study, we found that micro-RNA (miR)-190b is highly expressed in bladder cancer tissues and cells. Overexpression of miR-190b enhanced the proliferation, growth, migration and invasion capabilities, and angiogenesis of bladder cancer cells, whereas downregulation of miR-190b reversed these effects. Target prediction and dual luciferase reporter assays identified NLR family CARD domain containing 3 (NLRC3) as a potential target of miR-190b. Pathway analysis indicated that miR-190b promotes bladder cancer progression via the Wnt/β-catenin and mTOR signaling pathways. Taken together, our findings imply that miR-190b acts as a critical regulator for bladder cancer development by repressing NLRC3 and partly through the Wnt/β-catenin and mTOR pathways. Our study suggests that miR-190b may be served as a potential therapeutic target for bladder cancer treatment.
Insights
MicroRNA-190b is highly expressed in bladder cancer, promoting tumor growth and spread. Targeting this microRNA (miRNA) could offer new therapeutic strategies for bladder cancer.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Bladder cancer is a common malignancy with poorly understood molecular mechanisms.
- Identifying key molecular drivers is crucial for developing new bladder cancer therapies.
Purpose of the Study:
- To investigate the role of micro-RNA (miR)-190b in bladder cancer pathogenesis.
- To identify the molecular targets and pathways regulated by miR-190b in bladder cancer.
Main Methods:
- Analysis of miR-190b expression in bladder cancer tissues and cells.
- Functional assays (proliferation, migration, invasion, angiogenesis) upon miR-190b modulation.
- Target prediction and validation using dual luciferase reporter assays.
- Pathway analysis (Wnt/β-catenin, mTOR).
Main Results:
- miR-190b is significantly upregulated in bladder cancer.
- Overexpression of miR-190b enhances bladder cancer cell proliferation, migration, invasion, and angiogenesis.
- NLRC3 (NLR family CARD domain containing 3) was identified as a direct target of miR-190b.
- miR-190b promotes bladder cancer progression through Wnt/β-catenin and mTOR signaling pathways.
Conclusions:
- miR-190b is a key regulator in bladder cancer development and progression.
- miR-190b exerts its function by targeting NLRC3 and modulating Wnt/β-catenin and mTOR pathways.
- miR-190b represents a potential therapeutic target for bladder cancer treatment.
