Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Protective role of bicarbonate ringer in traumatic shock: A clinical and experimental study.

Current problems in surgery·2026
Same author

An Analyte-Derived Turn-On Fluorescent Nanozyme Sensor Enabled by a Programmable Light-Driven Cascade for o-Nitroaniline Detection.

Analytical chemistry·2026
Same author

A combination of QTL mapping and genome‑wide association study revealed key genes for heat tolerance in maize.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2026
Same author

Highly sensitive detection of ochratoxin A by fluorescence polarization assay based on rolling circle amplification.

Analytical and bioanalytical chemistry·2026
Same author

Cathepsin Inhibitor Suppresses the Growth of Ectopic Hepatocellular Carcinoma Tumors in Mouse Models.

ACS pharmacology & translational science·2026
Same author

Cell Probe Cocktail Enables Ratiometric miRNA Detection with Enhanced Sensitivity and an Extended Dynamic Range.

Analytical chemistry·2026

Related Experiment Video

Updated: Dec 30, 2025

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method

Published on: October 7, 2025

265

A New One-Pot Fluorescence Derivatization Strategy for Highly Sensitive MicroRNA Analysis.

Li Pan1, Huaisheng Zhang1, Jingjin Zhao1,2

  • 1Department of Chemistry and Biochemistry, Jackson State University, 1400 Lynch Street, Jackson, Mississippi, 39217, USA.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|January 19, 2020
PubMed
Summary

This study introduces a new fluorescence method for analyzing microRNAs (miRNAs). The technique enables sensitive detection of miRNAs in serum, aiding disease diagnostics.

Keywords:
RNA recognitiondyes/pigmentsfluorescence spectroscopymagnetic propertiessynthetic methods

More Related Videos

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
14:15

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Published on: November 18, 2014

12.2K
Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

33.7K

Related Experiment Videos

Last Updated: Dec 30, 2025

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method

Published on: October 7, 2025

265
Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
14:15

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Published on: November 18, 2014

12.2K
Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

33.7K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Analytical Chemistry

Background:

  • MicroRNAs (miRNAs) regulate gene expression and are implicated in human diseases.
  • Accurate miRNA analysis is crucial for disease diagnostics.
  • Current methods for miRNA detection can be limited in sensitivity or complexity.

Purpose of the Study:

  • To develop a novel, sensitive, and efficient method for microRNA analysis.
  • To investigate the mechanism of a new fluorescence derivatization reaction for miRNAs.
  • To establish a quantitative assay for miRNA determination in biological samples.

Main Methods:

  • A one-pot fluorescence derivatization strategy using 2-chloroacetaldehyde (CAA).
  • Exploration of the reaction mechanism via liquid chromatography, fluorescence spectroscopy, and mass spectrometry.
  • Development of a high-performance liquid chromatography (HPLC) method coupled with magnetic solid-phase extraction (MSPE).

Main Results:

  • Formation and detachment of highly fluorescent N⁶-ethenoadenine (ϵ-adenine) from miRNA sequences.
  • Experimental evidence for the stabilization of oxocarbenium intermediates by ϵ-adenine and hydrogen bonding, enhancing depurination and derivatization.
  • A sensitive HPLC-MSPE method capable of quantifying miRNAs at sub-picomolar levels in serum.

Conclusions:

  • The novel derivatization strategy provides an effective mechanism for miRNA analysis.
  • The developed HPLC-MSPE method offers a facile and sensitive approach for quantitative miRNA determination.
  • This technique holds promise for advancing miRNA-based disease diagnostics using serum samples.