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Updated: Dec 30, 2025
An Improved Method for the Preparation of Type I Collagen From Skin
Published on: January 21, 2014
[Effect of genipin pretreatment on type Ⅰ collagen mineralization]
Tianyi Gu1, Jing Shuai1, Chaoqun Chen2
1School of Stomatology, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Objective:
To investigate the effects of bio-crosslinker genipin pretreatment on type Ⅰ collagen mineralization.
Methods:
Type Ⅰ collagen gels were prepared and pretreated with 0.5wt%genipin (experimental group) and deionized water (control group) for 2 h, respectively. The pretreated products were subjected to Fourier transform infrared spectroscopy (FT-IR). Reconstituted collagen fibrils were pretreated with genipin or deionized water for 2 h and were mineralized for 4 h. The collagen density and mineralization degree were examined with transmission electron microscopy (TEM) and analyzed with ImageJ software. Then scanning electron microscopy (SEM) and TEM were used to observe the mineralization of cross-linked demineralized dentin collagen.
Results:
FT-IR spectrum showed that the genipin was crosslinked with collagen. TEM observation and ImageJ results showed that after 4 h mineralization, the mineralization effect of 0.5wt% genipin group was significantly better than that of the control group[(73.3±5.3)%vs.(7.4±3.5)%,P<0.01]. TEM and SEM observation showed that the mineralization rate of type Ⅰ collagen and demineralized dentin pretreated with genipin were significantly faster than that of the control group.
Conclusions:
The study demonstrates that 0.5 wt% concentration of genipin can significantly promote the mineralization of type Ⅰ collagen.
