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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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CIRCLE-Seq for Interrogation of Off-Target Gene Editing
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Probing 3D genome by CRISPR/Cas9.

Pei Feng Liu1, Qiang Wu1

  • 1Center for Comparative Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Institute of Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, China.

Yi Chuan = Hereditas
|January 21, 2020
PubMed
Summary
This summary is machine-generated.

The CRISPR/Cas9 gene editing tool and its advanced DNA-fragment editing technologies are powerful for studying the three-dimensional (3D) genome. These methods help investigate chromatin organization and its effects on gene regulation and cell development.

Keywords:
3D genomeCRISPR/Cas9 systemCas9 endonuclease cleavage mechanismDNA fragment editingchromosome rearrangement

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Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • The CRISPR/Cas9 system offers efficient and cost-effective genome editing across various organisms.
  • Three-dimensional (3D) genomics integrates chromosome conformation capture, next-generation sequencing, and super-resolution microscopy.
  • Understanding 3D genome structure is crucial for gene expression, cell development, and spatial organization.

Purpose of the Study:

  • To review the applications and challenges of CRISPR/Cas9 and its derivatives in 3D genome research.
  • To highlight the potential of these gene editing tools for investigating higher-order chromatin organization.
  • To provide references and suggest future research directions in the field.

Main Methods:

  • CRISPR/Cas9 gene editing technology.
  • Derivative technologies for DNA-fragment editing.
  • Integration with 3D genome analysis techniques (e.g., chromosome conformation capture, live imaging).

Main Results:

  • CRISPR/Cas9 enables precise manipulation of genomic elements within the 3D genome.
  • These tools facilitate the study of structural variations and their impact on gene regulation.
  • The technologies are valuable for exploring chromatin dynamics and spatial genome organization.

Conclusions:

  • CRISPR/Cas9 and related technologies are essential toolboxes for 3D genome research.
  • These tools offer significant opportunities for advancing our understanding of chromatin architecture.
  • Further research is needed to overcome challenges and fully leverage these technologies in 3D genomics.