Comparison of different images in gross target volume delineating on VX2 nasopharyngeal transplantation tumor models

Background: To determine the optimum conditions for diagnosis of nasopharyngeal carcinoma, we established VX2 rabbit model to delineate gross target volume (GTV) in different imaging methods. Methods: The orthotopic nasopharyngeal carcinoma (NPC) was established in sixteen New Zealand rabbits. After 7-days inoculation, the rabbits were examined by CT scanning and then sacrificed for pathological examination. To achieve the best delineation, different GTVs of CT, MRI, ¹⁸F-FDG PET/CT, and ¹⁸F-FLT PET/CT images were correlated with pathological GTV (GTVp). Results: We found 45% and 60% of the maximum standardized uptake value (SUV_max) as the optimal SUV threshold for the target volume of NPC in ¹⁸F-FDG PET/CT and ¹⁸F-FLT PET/CT images, respectively (GTV_FDG45% and GTV_FLT60%). Moreover, the GTV_MRI and GTV_CT were significantly higher than the GTVp (P ≤ 0.05), while the GTV_FDG45% and especially GTV_FLT60% were similar to the GTVp (R = 0.892 and R = 0.902, respectively; P ≤ 0.001). Conclusions: Notably, the results suggested that ¹⁸F-FLT PET/CT could reflect the tumor boundaries more accurately than ¹⁸F-FDG PET/CT, MRI and CT, which makes ¹⁸F-FLT PET-CT more advantageous for the clinical delineation of the target volume in NPC.