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TRIM34 localizes to the mitochondria and mediates apoptosis through the mitochondrial pathway in HEK293T cells

Xinye An1, Bing Ji1, Dakang Sun2

  • 1Laboratory of Clinical Medicine, Binzhou, 256603, China.

Heliyon
|January 21, 2020
PubMed

Insights

Tripartite motif 34 (TRIM34) localizes to mitochondria and induces apoptosis in human embryonic kidney cells. This protein reduces cell viability by disrupting mitochondrial membrane potential and releasing cytochrome c.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Immunology

Background:

  • Tripartite motif 34 (TRIM34) is an interferon-induced protein.
  • The subcellular localization and biological functions of TRIM34 are not well understood.

Purpose of the Study:

  • To investigate the subcellular localization of TRIM34.
  • To elucidate the biological function of TRIM34, particularly its role in cell viability and apoptosis.

Main Methods:

  • Confocal microscopy was used to determine TRIM34 localization.
  • Western blotting analyzed TRIM34 presence in mitochondrial fractions.
  • Cell viability was assessed using the CCK-8 assay.
  • Apoptosis and cell cycle distribution were analyzed by flow cytometry.

Main Results:

  • TRIM34 was found in the cytoplasm and mitochondria of HEK293T cells.
  • TRIM34 significantly reduced HEK293T cell viability.
  • TRIM34 induced apoptosis in HEK293T cells.
  • TRIM34 promoted apoptosis through loss of mitochondrial membrane potential and cytochrome c release.

Conclusions:

  • TRIM34 localizes to mitochondria and induces apoptosis in HEK293T cells.
  • TRIM34-induced apoptosis is mediated by mitochondrial membrane potential depolarization.
  • TRIM34 may play a significant role in cellular regulation and stress responses.
Keywords:
ApoptosisCell biologyColocalizationImmunologyMicrobiologyMitochondriaMolecular biologyProteinsTripartite motif 34 (TRIM34)

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