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Updated: Dec 30, 2025
A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays
Published on: August 26, 2013
Combined High Throughput Screening with QSAR Analysis Unravelling Potential Glyoxalase-I Inhibitors
Mahmoud A Al-Sha'er1, Qosay A Al-Balas2, Mohammad A Hassan2
1Faculty of Pharmacy, Zarqa University, Zarqa 13132, Jordan.
Aims:
Discovery of new Glo-I inhibitors as potential anticancer agents.
Background:
Glyoxalase system is ubiquitous system in human cells which has been examined thoroughly for its role in cancerous diseases. It performs detoxifying endogenous harmful metabolites, mainly methylglyoxal (MG) into non-toxic bystanders.
Objective:
Structure based model Hypo(2ZA0_2_02) combined with 3D-QSAR modeling were applied to predict glyoxalase I inhibition and to explain their activity.
Methods:
Currently, high throughput screening approach was used to investigate the activity of inhouse database composed of 205 compounds.
Results:
15 compounds were found active as glyoxalase I inhibitors. The 15 candidates showed more than 50% inhibition with low micromolar IC50 ranges between 5.0 to 42.0 μM.
Conclusion:
They have been successfully mapped and fitted the Hypo(2ZA0_2_02) model which explain the presence of anti-glyoxalase I activity. This model could be used in future for further development of new and novel glyoxylase I inhibitors.
