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Related Concept Videos

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
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Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
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Modern Molecular Taxonomy

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Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
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Updated: Dec 30, 2025

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
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Progress and applications of single-cell sequencing techniques.

Aimaiti Yasen1, Abudusalamu Aini2, Hui Wang3

  • 1State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Xinjiang Medical University, 393 Xin Yi Road, Urumqi 830011, Xinjiang Uyghur Autonomous Region, People's Republic of China; The first affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uyghur Autonomous Region, People's Republic of China; Department of Hepatobiliary and Hydatid Disease, Digestive and Vascular Surgery Center, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uyghur Autonomous Region, People's Republic of China.

Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases
|January 21, 2020
PubMed
Summary

Single-cell sequencing (SCS) analyzes individual cell genetic and protein data. This powerful method aids in understanding disease mechanisms and advancing clinical applications.

Keywords:
ApplicationEpigenetic sequencingSingle-cell sequencingWhole-genome sequencingWhole-transcriptome sequencing

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Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • Single-cell sequencing (SCS) is an advanced technique for analyzing genetic and protein variations between individual cells.
  • It enables the study of microorganisms that are challenging to cultivate, revealing their specific roles within microenvironments.

Purpose of the Study:

  • To summarize the methodologies and diverse application areas of single-cell sequencing.
  • To provide insights into the potential future clinical utility of SCS.

Main Methods:

  • Whole genome, transcriptome, and epigenome sequencing of individual cells.
  • Analysis of cellular heterogeneity in disease pathogenesis.

Main Results:

  • SCS can elucidate complex mechanisms in disease occurrence and progression.
  • It offers improved disease diagnosis, prognosis prediction, and monitoring of therapeutic drug effects.

Conclusions:

  • SCS is valuable for studying embryonic and organ development, immune system functions, and cancer progression.
  • Applications extend to parasitic/infectious diseases, stem cell research, antibody screening, and therapeutic R&D.