An innovative cell imaging by beet root extracted pigment

Area of Science:

• Biotechnology
• Cell Biology
• Organic Chemistry

Background:

• Fluorescent dyes are crucial for visualizing cellular structures and functions.
• Existing dyes can be expensive or difficult to extract and preserve.
• There is a need for efficient, accessible, and stable fluorescent probes for cell imaging.

Purpose of the Study:

• To discover and characterize a novel, water-soluble fluorescent dye from beet root.
• To evaluate the dye's efficacy in various cell imaging techniques.
• To assess the dye's stability and internalization time in human cells.

Main Methods:

• Extraction and purification of the Beet-root extracted water-soluble fluorescent (BREWSF) dye.
• Characterization using IR, UV-Vis, NMR, mass, and fluorescence spectroscopy.
• Application in fluorescence microscopy, flow cytometry, and confocal imaging of human cells.

Main Results:

• Successful extraction of a water-soluble fluorescent dye from beet root.
• Comprehensive characterization confirming the dye's properties.
• Excellent performance in multiple cell imaging modalities with rapid internalization.
• Demonstrated stability across a range of pH levels.

Conclusions:

• The BREWSF dye is an efficient, cost-effective, and stable fluorescent probe for cell imaging.
• Its ease of extraction and preservation makes it a valuable tool for biological research.
• The dye shows significant potential for advancing cellular visualization techniques.