Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.5K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Functional xenogeneic hematopoietic cells maintaining donor-dominant identity and immune tolerance enable therapy.

Experimental hematology & oncology·2026
Same author

Multi-omics uncovers immune-modulatory molecules in plasma contributing to resistance exercise-ameliorated locomotor disability after incomplete spinal cord injury.

Genome medicine·2025
Same author

Spliceosome inhibitor induces human hematopoietic progenitor cell reprogramming toward stemness.

Experimental hematology & oncology·2022
Same author

Trajectory of chemical cocktail-induced neutrophil reprogramming.

Journal of hematology & oncology·2020
Same author

Hematopoietic Reprogramming Entangles with Hematopoiesis.

Trends in cell biology·2020
Same author

Conserved Tao Kinase Activity Regulates Dendritic Arborization, Cytoskeletal Dynamics, and Sensory Function in <i>Drosophila</i>.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2020

Related Experiment Video

Updated: Dec 30, 2025

Chemogenetic Regulation in Reprogrammed Stem Cell-derived Precursor Cells in Treating Neurodegenerative Diseases
09:44

Chemogenetic Regulation in Reprogrammed Stem Cell-derived Precursor Cells in Treating Neurodegenerative Diseases

Published on: May 2, 2025

557

Reprogramming of Fibroblasts to Neural Stem Cells by a Chemical Cocktail.

Chuijin Wei1, Shumin Xiong1, Lin Cheng2

  • 1State Key Laboratory of Medical Genomics, Shanghai Institute of Hematology, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Methods in Molecular Biology (Clifton, N.J.)
|January 22, 2020
PubMed
Summary

Chemical compounds can reprogram mouse fibroblasts into neural stem cells (NSCs). This method offers advantages over traditional cell reprogramming techniques for generating functional cells.

Keywords:
Chemical compoundsDirect cell reprogrammingMouse fibroblastsNeural stem cellsTransdifferentiation

More Related Videos

Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells
13:58

Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells

Published on: July 29, 2015

16.0K
In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes
11:42

In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes

Published on: June 10, 2021

5.3K

Related Experiment Videos

Last Updated: Dec 30, 2025

Chemogenetic Regulation in Reprogrammed Stem Cell-derived Precursor Cells in Treating Neurodegenerative Diseases
09:44

Chemogenetic Regulation in Reprogrammed Stem Cell-derived Precursor Cells in Treating Neurodegenerative Diseases

Published on: May 2, 2025

557
Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells
13:58

Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells

Published on: July 29, 2015

16.0K
In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes
11:42

In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes

Published on: June 10, 2021

5.3K

Area of Science:

  • Stem cell biology
  • Chemical biology
  • Neuroscience

Background:

  • Cell fate conversion is crucial for regenerative medicine.
  • Chemical reprogramming offers advantages over genetic methods.
  • Neural stem cells (NSCs) are vital for neurological research and therapies.

Purpose of the Study:

  • To establish a protocol for chemically inducing direct reprogramming of fibroblasts into NSCs.
  • To demonstrate the efficacy of a chemical cocktail for NSC generation.

Main Methods:

  • Utilizing a specific cocktail of chemical compounds.
  • Applying the chemical treatment to mouse fibroblast cultures.
  • Characterizing the resulting cells for NSC markers and functionality.

Main Results:

  • Successfully converted mouse fibroblasts into neural stem cells (NSCs).
  • The generated NSCs exhibited characteristic markers and potential for differentiation.
  • The chemical reprogramming protocol proved efficient and reproducible.

Conclusions:

  • Chemical induction is a viable and advantageous strategy for generating NSCs from fibroblasts.
  • This protocol provides a valuable tool for NSC research and potential therapeutic applications.