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Related Experiment Videos

A method for concentrating cerebrospinal fluid cells.

H W Kölmel

    Acta Cytologica
    |January 1, 1977
    PubMed
    Summary

    Rapid changes in cerebrospinal fluid (CSF) cell morphology necessitate optimal cell preparation. Forced spontaneous sedimentation offers the best cytomorphologic results for CSF cell evaluation.

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    Area of Science:

    • Cytopathology
    • Cell Biology
    • Biomedical Sciences

    Background:

    • Cerebrospinal fluid (CSF) cell morphology changes rapidly in vitro.
    • Optimal cytologic evaluation requires specialized cell preparation techniques.
    • Existing methods may not adequately preserve cell morphology for accurate analysis.

    Purpose of the Study:

    • To describe a simple and effective method for concentrating CSF cells.
    • To present two variations of a cell concentration technique.
    • To demonstrate the suitability of the method for specific cell types and experimental conditions.

    Main Methods:

    • A simple cell concentration method using forced spontaneous sedimentation.
    • Two variations: direct sedimentation onto a microscope slide or onto a membrane filter.
    • Sedimentation facilitated by absorbent paper.

    Main Results:

    • Forced spontaneous sedimentation yields optimal cytomorphologic results.
    • The method is adaptable based on cell content.
    • Effective for concentrating cultured cells and CSF cells, especially when incubated with tracers.

    Conclusions:

    • The described method provides excellent cytomorphologic preservation for CSF cells.
    • This technique is valuable for accurate cytologic evaluation of CSF and cultured cells.
    • Facilitates reliable analysis of cells, including those exposed to tracers.

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