Paenibacillus ottowii sp. nov. isolated from a fermentation system processing bovine manure

Area of Science:

• Microbiology
• Bacteriology
• Environmental Science

Background:

• The genus Paenibacillus comprises diverse bacteria found in various environments.
• Understanding microbial diversity is crucial for ecological and biotechnological applications.

Purpose of the Study:

• To characterize and classify a novel bacterial isolate, strain MS2379^T, from a bovine manure fermentation system.
• To determine the phylogenetic and chemotaxonomic distinctiveness of strain MS2379^T.

Main Methods:

• Phylogenetic analysis using 16S rRNA and rpoB gene sequences.
• DNA-DNA hybridization for genetic relatedness assessment.
• Chemotaxonomic analyses including fatty acid profiling, peptidoglycan analysis, and polar lipid identification.
• Biochemical tests such as the Voges-Proskauer reaction.
• Determination of growth parameters (temperature, pH, NaCl tolerance).

Main Results:

• Strain MS2379^T, isolated from a bovine manure fermentation system, showed phylogenetic relatedness to Paenibacillus polymyxa, P. jamilae, and P. peoriae.
• DNA-DNA hybridization revealed low relatedness (<33%) to closely related species, supporting its novelty.
• Chemotaxonomic and biochemical analyses, including a negative Voges-Proskauer reaction, distinguished MS2379^T from known Paenibacillus species.
• The G+C content was 45.9 mol%, and optimal growth occurred at 30°C between pH 6-10 and up to 3% NaCl.

Conclusions:

• Strain MS2379^T represents a novel species within the Paenibacillus genus.
• The proposed name for this new species is Paenibacillus ottowii sp. nov., with MS2379^T as the type strain.
• This discovery contributes to the understanding of bacterial diversity in anaerobic digestion systems.