Related Experiment Video
Updated: Dec 30, 2025
The Creation of a Rat Model for Osteosarcopenia via Ovariectomy
Published on: February 21, 2025
Ovariectomy and timing of impaired maxillary alveolar bone regeneration: An experimental study in rats
Hyun Ju Kim1, Kyoung-Hwa Kim2, Yong-Moo Lee2
1Department of Periodontics, Seoul National University Dental Hospital, Seoul, Korea.
Background:
The aim of this study was to seek the critical time for impairment of alveolar bone regeneration after ovariectomy (OVX) in rats.
Methods:
A total of 32 female rats were used. Test group rats were divided into a 2M group (n = 8), a 3M group (n = 8) and a 4M group (n = 8) according to the duration from OVX to defect creation. Bilateral OVX was performed in all test groups, and a sham operation was performed in the control group (n = 8). Drill-hole defects (1.5 mm diameter, 2 mm length) were created on both sides of the maxilla. All rats were euthanized 2 and 4 weeks after the surgery. Microcomputed tomographic (micro-CT), histological, and histomorphometric analyses and in vitro experiments were performed.
Results:
The 4M group showed significantly less new bone formation and a lower bone mineral density than the other groups in the micro-CT analysis. The histomorphometric analysis also revealed that the 4M group showed significantly less new bone formation than the control and 2M groups. The rats in the 4M group showed significantly higher alkaline phosphatase expression levels and a larger number of calcified nodules than rats in the other groups, whereas osteoclastic activity was significantly lower in the 4M group than in the other groups.
Conclusions:
The critical time for impairment of alveolar bone regeneration was 4 months after OVX in rats.
