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Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents
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Antiarrhythmic effect of crotonoside by regulating sodium and calcium channels in rabbit ventricular myocytes

Zhipei Liu1, Yuzhong Jia2, Lv Song2

  • 1Cardio-Electrophysiological Research Laboratory, Medical College of Wuhan University of Science and Technology, Wuhan 430065, China; Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Medical College of Wuhan University of Science and Technology, Wuhan 430065, China.

Life Sciences
|January 22, 2020
PubMed
Abstract

Insights

Crotonoside (Cro) demonstrates antiarrhythmic effects by inhibiting key ion currents in heart cells. This compound effectively reduces ventricular arrhythmias in vivo, suggesting therapeutic potential.

Area of Science:

  • Cardiovascular Pharmacology
  • Electrophysiology
  • Pharmacology

Background:

  • Cardiac arrhythmias pose a significant health risk.
  • Identifying novel antiarrhythmic agents is crucial for clinical management.

Purpose of the Study:

  • To investigate the antiarrhythmic effects of crotonoside (Cro).
  • To elucidate the mechanisms underlying Cro's action on cardiac ion channels and action potentials.

Main Methods:

  • Whole-cell patch-clamp techniques were employed on isolated rabbit ventricular myocytes.
  • Effects of Cro on action potentials and transmembrane ion currents were assessed.
  • In vivo studies evaluated the efficacy of Cro against aconitine-induced ventricular arrhythmias.

Main Results:

  • Crotonoside concentration-dependently reduced action potential upstroke velocity (Vmax) and action potential duration (APD).
  • Cro inhibited peak sodium current (INa) and L-type calcium current (ICAL) with IC50 values of 192 μmol/L and 159 μmol/L, respectively.
  • Cro abolished late sodium current (INaL) and suppressed both early and delayed afterdepolarizations, significantly reducing in vivo ventricular arrhythmias.

Conclusions:

  • Crotonoside effectively inhibits INa, INaL, and ICAL in ventricular myocytes.
  • Crotonoside exhibits significant antiarrhythmic potential, warranting further investigation for therapeutic applications.
Keywords:
CrotonosideL-type calcium currentLate sodium currentPeak sodium currentVentricular arrhythmias

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