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New biotin-conjugated antisera for quantitation of Mycoplasma gallisepticum-specific immunoglobulin A in chicken
E K Barbour1, J A Newman, V Sivanandan
1Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.
The biotinylation of goat anti-alpha-chains of chicken immunoglobulin A (IgA), suitable for use in an avidin-biotin enzyme-linked immunosorbent assay, is described. The optimum conditions for the use of the developed conjugate in determining local and systemic IgA specific to Mycoplasma gallisepticum in chickens were established.
The biotinylation of goat anti-alpha-chains of chicken immunoglobulin A (IgA), suitable for use in an avidin-biotin enzyme-linked immunosorbent assay, is described. The optimum conditions for the use of the developed conjugate in determining local and systemic IgA specific to Mycoplasma gallisepticum in chickens were established.