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Published on: March 28, 2017
Staphylococcus saprophyticus Proteomic Analyses Elucidate Differences in the Protein Repertories among Clinical
Karla Christina Sousa Silva1, Lana O'Hara Souza Silva1, Guilherme Algusto Alves Silva1
1Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia-GO 74690-900, Brazil.
Abstract:
Staphylococcus saprophyticus is a Gram-positive and coagulase negative cocci that composes the skin microbiota and can act as an opportunistic agent causing urinary tract infections, being more frequent in sexually active young women. The ability of a pathogen to cause infection in the host is associated to its ability to adhere to host cells and to survive host immune defenses. In this work, we presented the comparative proteomic profile of three S. saprophyticus strains. It was possible to characterize differences in the proteome content, specially related to expression of virulence factors. We compiled this data and previous data and we detected one strain (9325) possessing higher production and secretion of proteins related to virulence. Our results show that phenotypic, genotypic, and proteomic differences reflect in the ability to survive during interaction with host cells, since the 9325 strain presented a higher survival rate after macrophage interaction. In counterpart, the 7108 strain that possesses lower content of proteins related to virulence presented higher ability to form biofilm suggesting that this strain can be better adapted to persist in the host and in the environment. Our work describes, for the first time, proteomic flexibility among S. saprophyticus strains, reflecting in virulence and persistence.
Insights
This study reveals proteomic differences in Staphylococcus saprophyticus strains, impacting their virulence and persistence. One strain showed higher virulence, while another excelled at biofilm formation, highlighting strain variability.
Area of Science:
- Microbiology
- Proteomics
- Bacterial Pathogenesis
Background:
- Staphylococcus saprophyticus is an opportunistic pathogen causing urinary tract infections, particularly in young women.
- Pathogen virulence is linked to adherence and immune evasion capabilities.
- Understanding strain-level differences is crucial for infection control.
Purpose of the Study:
- To comparatively analyze the proteomic profiles of three S. saprophyticus strains.
- To investigate the relationship between proteomic variations, virulence, and host interaction.
- To explore strain-specific adaptations for persistence.
Main Methods:
- Comparative proteomic analysis of S. saprophyticus strains.
- Quantification and characterization of secreted proteins.
- Assessment of bacterial survival following macrophage interaction.
- Biofilm formation assays.
Main Results:
- Significant differences in proteome content, particularly virulence factors, were identified among strains.
- Strain 9325 exhibited higher production of virulence proteins and enhanced survival against macrophages.
- Strain 7108 showed lower virulence protein content but superior biofilm formation capacity.
- Proteomic flexibility correlates with differential virulence and persistence.
Conclusions:
- Proteomic variability exists among S. saprophyticus strains.
- This variability influences the bacteria's ability to cause infection and persist.
- The study provides novel insights into S. saprophyticus pathogenesis and adaptation.
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