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Updated: Dec 30, 2025

Synthesis and Purification of Iodoaziridines Involving Quantitative Selection of the Optimal Stationary Phase for Chromatography
Published on: May 16, 2014
Energy-efficient and environment-friendly method to prepare monodispersed silica stationary phases for simultaneous
Zhixia Huo1, Qian-Hong Wan1, Lei Chen1
1School of Pharmaceutical Science & Technology, Tianjin University, 92 Weijin Road, Tianjin, 300072, China.
Abstract:
An energy-efficient and environment-friendly approach to prepare porous monodispersed micro-sized silica particles with methyltrimethoxysilane (MTMS) as the precursor is described. The particles were synthesized by a two-step hydrolysis/condensation procedure, with post-synthetic aging and calcination for methyl group removal. They show uniform spherical morphology, narrow particle size distribution (D90/10, 1.2-1.6), tailored particle size (3, 5, 7 μm) and mesopore structure (10, 13 nm), which can be directly used as packing materials for HPLC without size classification. C18, sulfonate, and C18/sulfonate mixed-mode stationary phases were produced by a green vapor deposition method based on the synthesized silica particles. The newly synthesized C18 phase exhibits mechanical stability, kinetic behavior and separation performance expected from the commercial C18 column. The C18/sulfonate phase exhibits prominent mixed-mode retention behavior which can be applied to the simultaneous separation of multiple substances in the compound drugs.
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