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Related Experiment Video

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Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes
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Listeria monocytogenes Exploits Host Caveolin for Cell-to-Cell Spreading.

Aaron S Dhanda1, Connie Yu1, Katarina T Lulic1

  • 1Department of Biological Sciences, Centre for Cell Biology, Development, and Disease, Simon Fraser University, Burnaby, British Columbia, Canada.

Mbio
|January 23, 2020
PubMed
Summary

Listeria monocytogenes cell-to-cell spread relies on caveolin-1 endocytosis, a mechanism that bypasses the typical size limits for this pathway. This bacterial invasion utilizes caveolin-1 and epsin-1, despite lacking clathrin.

Keywords:
Listeria monocytogenesactinactin-based motilitycell-to-cell spreadingendocytosisepsin-1invaginationsmembrane protrusion

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Area of Science:

  • Cell Biology
  • Microbiology
  • Infectious Diseases

Background:

  • Listeria monocytogenes disseminates within host tissues through cell-to-cell transfer.
  • Bacterial cell-to-cell spread involves actin-rich membrane protrusions and host cell internalization.
  • The precise host internalization mechanism for Listeria monocytogenes remains incompletely understood.

Purpose of the Study:

  • To elucidate the host cell internalization mechanism utilized by Listeria monocytogenes during cell-to-cell transfer.
  • To investigate the role of caveolin endocytosis and associated proteins in Listeria monocytogenes spreading.
  • To determine if Listeria monocytogenes can overcome the theoretical size limitations of caveolin-mediated endocytosis.

Main Methods:

  • Depletion of caveolin-1 and other caveolar components in host cells.
  • Analysis of clathrin and clathrin-associated proteins at sites of bacterial invasion.
  • Experimental setup using isolated Listeria monocytogenes membrane protrusions to trigger host cell uptake.

Main Results:

  • Bacterial cell-to-cell transfer is significantly impaired in caveolin-1-depleted cells.
  • Caveolin-1, cavin-2, EHD2, and epsin-1 are involved in Listeria monocytogenes internalization.
  • Isolated bacterial protrusions can induce caveolin-1 recruitment and internalization in recipient cells, exceeding theoretical size limits.

Conclusions:

  • Caveolin-1-mediated endocytosis is essential for efficient Listeria monocytogenes cell-to-cell spreading.
  • The internalization process involves a subset of caveolar proteins and the clathrin-associated protein epsin-1.
  • Listeria monocytogenes utilizes a caveolin-based mechanism to exceed the classical size constraints of this endocytic pathway.