Microbial Community Analysis of Digested Liquids Exhibiting Different Methane Production Potential in Methane Fermentation of Swine Feces

Batch methane fermentation was conducted using seed sludge collected from six methane fermentation facilities. Swine feces were centrifuged and autoclaved, followed by its use as a substrate for methanogenesis. This "swine feces supernatant medium" facilitates the cultivation of the microbes of the seed sludge, sampling of the digested liquid using a syringe, and subculturing of the digested liquid in a subsequent medium using a syringe. Through 15 subcultures, digested liquids with high and low methane production potential were obtained, which were named "H-DS" and "L-DS," respectively. On the day 10 of cultivation, chemical oxygen demand (COD) of H-DS significantly decreased by 31% and that of L-DS did not differ significantly compared with that on the day 0 of cultivation. Acetic acid concentration of H-DS (1009 mg/L) was significantly lower than that of L-DS (2686 mg/L). These chemical characteristics indicate that organics decomposition in L-DS was not successful and suggest that H-DS has high relative abundance of bacteria decomposing organic matter and methanogen utilizing acetic acid compared with those in L-DS. Microbial community analysis revealed that Shannon index of H-DS was significantly higher than that of L-DS, and the relative abundance of acetogenic bacteria (e.g., Syntrophomonas) and acetic acid-utilizing methanogen (Methanosarcina) in H-DS was significantly higher than that in L-DS. Thus, the high methane production potential of H-DS might be attributable to the smooth flow from acetogenesis to methanogenesis step in the methane fermentation, compared with the case of L-DS.