Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

LPAR4 mediates resistance to interferon-induced stress in soft tissue sarcoma.

Science advances·2026

Same author

Cuttlebone-inspired PMIA membranes with symmetric porous structure for enhanced mechanical stability of ultrafiltration.

Materials horizons·2026

Same author

Nanoporous PVDF-HFP/PMIA Membrane with Heat-Resistance towards the Gel Polymer Electrolyte of Lithium Metal Batteries.

ACS applied materials & interfaces·2026

Same author

Negatively charged Janus membranes with robust pore anti-wettability for stable membrane distillation of seawater.

Nanoscale·2025

Same author

An Ammonium Polyphosphate Nanoparticle-Based Flame-Retardant Separator Enhances Safety of Lithium-Metal Batteries.

Small methods·2025

Same author

The deubiquitinating enzyme Cezanne stabilizes BRCA1 by counteracting APC/C and Ube2S-dependent Lys11-linked ubiquitination.

PLoS biology·2025

Search research articles

Related Experiment Video

Updated: Dec 30, 2025

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Microfluidics-Based Single-Cell Protrusion Analysis for Screening Drugs Targeting Subcellular Mitochondrial

Pengchao Zhang^1,2, Jun Yao, Bin Wang

¹Department of Nanomedicine , Houston Methodist Research Institute , 6670 Bertner Avenue , Houston , Texas 77030 , United States.

Analytical Chemistry

|January 23, 2020

Summary

Mitochondria are crucial for cancer cell protrusions, which drive migration. A new microfluidic platform quantifies how mitochondrial trafficking impacts protrusion formation, aiding anticancer drug development.

More Related Videos

Generation of Heterogeneous Drug Gradients Across Cancer Populations on a Microfluidic Evolution Accelerator for Real-Time Observation

Generation of Heterogeneous Drug Gradients Across Cancer Populations on a Microfluidic Evolution Accelerator for Real-Time Observation

Published on: September 19, 2019

Analysis of Cancer Cell Invasion and Anti-metastatic Drug Screening Using Hydrogel Micro-chamber Array HMCA-based Plates

Analysis of Cancer Cell Invasion and Anti-metastatic Drug Screening Using Hydrogel Micro-chamber Array HMCA-based Plates

Published on: October 25, 2018

Related Experiment Videos

Last Updated: Dec 30, 2025

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Generation of Heterogeneous Drug Gradients Across Cancer Populations on a Microfluidic Evolution Accelerator for Real-Time Observation

Generation of Heterogeneous Drug Gradients Across Cancer Populations on a Microfluidic Evolution Accelerator for Real-Time Observation

Published on: September 19, 2019

Analysis of Cancer Cell Invasion and Anti-metastatic Drug Screening Using Hydrogel Micro-chamber Array HMCA-based Plates

Analysis of Cancer Cell Invasion and Anti-metastatic Drug Screening Using Hydrogel Micro-chamber Array HMCA-based Plates

Published on: October 25, 2018

Area of Science:

Cell Biology
Biophysics
Cancer Research

Background:

Cancer cell migration is essential for metastasis and is guided by cell protrusions.
Subcellular mitochondrial localization and trafficking are implicated in protrusion formation, but quantitative data are lacking.

Purpose of the Study:

To develop a high-throughput microfluidic platform for quantitative, single-cell analysis of cell protrusion formation regulated by subcellular mitochondrial trafficking.
To investigate the role of mitochondria in cell protrusion generation during cancer cell migration.

Main Methods:

High-throughput microfluidic platform for quantitative single-cell analysis.
Gene expression profiling and mitochondrial staining of isolated cell protrusions.
Inhibition of subcellular mitochondrial trafficking.

Main Results:

Mitochondria are present in high numbers within cell protrusions.
Inhibiting subcellular mitochondrial trafficking significantly suppressed cell protrusion formation.
The microfluidic platform allows for precise evaluation of mitochondria-specific therapeutic drugs' effects on cell protrusion formation.

Conclusions:

Subcellular mitochondrial trafficking is critical for cancer cell protrusion formation and migration.
The developed microfluidic platform offers a valuable tool for high-throughput screening of anticancer agents targeting mitochondrial function.
This approach has potential clinical utility in developing novel anticancer therapies.