Related Experiment Video
Updated: Dec 30, 2025

CAM-Delam Assay to Score Metastatic Properties by Quantifying Delamination and Invasion Capacity of Cancer Cells
Published on: June 2, 2022
Artemin promotes proliferation and metastasis in human laryngeal squamous cell carcinoma
Chao-Bing Gao1, Dong Wang1, Yang Wang1
1Department of Otorhinolaryngology, Head and Neck Surgery, The First Affiliated Hospital of AnHui Medical University Hefei, Anhui, China.
Abstract:
Laryngeal squamous cell carcinoma (LSCC) is the main type of human laryngeal cancer which is one of the most common malignant head and neck tumors and the outcomes of LSCC patients are always poor. The intrinsic molecular mechanisms in initiation, development, growth and metastasis of LSCC remain unclear. Further researches are necessary and urgent. In this article, we examined artemin (ARTN) promoted both cell proliferation and metastasis of human LSCC cells Hep-2 by siRNA mediated ARTN knocking down using MTT assay, cell migration assay and cell invasion assay. Moreover, we examined the expression level of ARTN in LSCC tissues was much higher than that in benign laryngeal polyp tissues. In addition, ARTN was identified as a direct target of miR-223 and miR-223 suppressed the expression of ARTN in LSCC cells. Supplement to our former study, we demonstrated ARTN was oncogenic both in vitro and in clinical tissues. As a result, ARTN could be used as a potential therapeutic target for human LSCC.
More Related Videos
06:08Establishment and Characterization of Patient-Derived Xenograft Models of Anaplastic Thyroid Carcinoma and Head and Neck Squamous Cell Carcinoma
Published on: June 2, 2023
10:32Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs
Published on: December 19, 2019
Related Concept Videos
Abnormal Proliferation
Adaptive Mechanisms in Cancer Cells
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
Metastasis
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
Mitogens and the Cell Cycle