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Membrane type-2 matrix metalloproteinases improve the progression of renal cell cancer

Yiming Chen1, Hao Lu1, Ding Tao1

  • 1Department of Urology, The Third Affiliated Hospital of Soochow University 185 Juqian Street, Changzhou 213003, People's Republic of China.

International Journal of Clinical and Experimental Pathology
|January 23, 2020
PubMed

Insights

Matrix metalloproteinases (MT2-MMP) promote renal cell carcinoma (RCC) invasion and growth. Suppressing MT2-MMP in ACHN cells reduced invasion, adhesion, and proliferation, while inhibiting apoptosis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Matrix metalloproteinases (MMPs) degrade extracellular matrix, aiding tumor invasion and metastasis.
  • Membrane type-2 MMP (MT2-MMP) is implicated in cancer progression, but its role in renal cell carcinoma (RCC) is unclear.

Purpose of the Study:

  • To investigate the role of MT2-MMP in the progression of human renal cell carcinoma (RCC).

Main Methods:

  • MT2-MMP expression was suppressed and overexpressed in ACHN renal cancer cells.
  • MMP-2 expression, cell adhesion, invasion, proliferation, and apoptosis were analyzed.
  • In vitro tumor growth was assessed via clone formation assays.

Main Results:

  • MT2-MMP expression levels varied among RCC cell lines, with ACHN cells showing high MT2-MMP.
  • MT2-MMP suppression or overexpression modulated MMP-2 expression in ACHN cells.
  • MT2-MMP significantly enhanced ACHN cell proliferation, adhesion, invasion, and clone formation, while inhibiting apoptosis.

Conclusions:

  • MT2-MMP promotes renal cell carcinoma (RCC) invasion and adhesion by activating MMP-2.
  • MT2-MMP stimulates tumor growth in renal cancer by increasing proliferation and inhibiting apoptosis.
Keywords:
MT2-MMPRenal cell carcinomaadhesioninvasionproliferation

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