Role of sex hormone on morphological and histological changes in benign prostatic hypertrophy rats

The prostate, the key secondary male reproductive organ, serves an important function of alkalizing seminal fluid and protecting genetic information in the acidity of the vaginal tract. As males age, the most common urologic condition manifests as an enlargement of the prostate known as benign prostatic hypertrophy (BPH). The purpose of this study is to examine the relationship between hormonal regulation and the morphological changes in BPH. Furthermore, we examine whether the ion-transport pump, H-K-ATPase (HKA), mediates such hormonal regulation. The experiments were designed to test the effects of the primary male androgen, testosterone propionate (TP), as well as the female hormone, estradiol (E2).

The rats were divided into three groups; control group, TP group, and TP+E2 group. Both the TP and the E2 were diluted in vegetable oil and covered to eliminate light exposure. A subcutaneous injection of TP at 3 mg/mL was administered to induce BPH in each rat. After 6 weeks of TP-induced BPH, we divided these rats into two groups. In one group of BPH rats, we injected 60 µg of E2, and in another group of BPH rats, we injected 120 µg of E2 subcutaneously. The rats were sacrificed under anesthesia, and the prostate specimens were dissected. The rat's body weight and the prostate tissue weight were measured as the organ quotient.

The data indicate significant hypertrophy of the luminal cells in rats with 3 mg TP compared to the control (524.542 ± 4.637 vs. 350.583 ± 1.996, P-value < 0.005). Whereas, the group with 60 µg E2 on TP-induced BPH showed significant inhibitory effects compared to TP-induced BPH (385.571 ± 7.265 vs. 524.542 ± 4.637, P-value < 0.005). The experimental group with 120 µg E2 on TP-induced BPH also showed significant inhibitory effects compared to TP-induced BPH (465.857 ± 8.259 vs. 524.542 ± 4.637, P-value < 0.005). The inhibitory effects of the 60 µg E2 group were more significant than the inhibitory effects of the 120 µg E2 group (385.571 ± 7.265 vs. 465.857 ± 8.259, P-value < 0.005), suggesting the importance of maintaining a proper E2:TP ratio. Western blot analysis shows up-regulation of specific bands for HKA alpha subunit at ~97 kDa for TP-induced BPH and down-regulation of HKA in the TP+E2 treatment groups.

The results show that TP induces benign prostate hypertrophy. Whereas, E2 is shown to inhibit BPH; the effect of E2 inhibition on BPH requires the optimal ratio between E2 and TP. If such a ratio is not reached, then BPH inhibition will not occur or will be less effective by E2. Both the induction and inhibition of hypertrophic cells suggest that the prostate is under hormonal regulation. The proper E2:TP ratio plays a crucial role in the pathogenesis of BPH. The ratio of E2:TP may lead to new approaches to preventing and treating BPH disease in the future.