Related Experiment Video

Updated: Dec 30, 2025

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

8.8K

CAT104 silence behaves as a tumor suppressor in human leukemia cells by down regulating miR-182 expression

Chengfang Zhang1, Guanli Song2, Guanbo Song3

  • 1Department of Clinical Laboratory, Jining No. 1 People's Hospital Jining 272011, Shandong, China.

International Journal of Clinical and Experimental Pathology
|January 23, 2020
PubMed
Abstract

Insights

Long non-coding RNA CAT104 and microRNA 182 are key players in acute myeloid leukemia (AML) tumorigenesis. Silencing CAT104 inhibits AML cell growth and metastasis by downregulating miR-182, which acts as an oncogene by upregulating ZEB1.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) are implicated in acute myeloid leukemia (AML) development.
  • Investigating the roles of lncRNA-CAT104 and miR-182 in AML pathogenesis is crucial.

Purpose of the Study:

  • To elucidate the functions and underlying mechanisms of lncRNA-CAT104 and miR-182 in AML.
  • To determine the relationship between CAT104, miR-182, and ZEB1 in leukemia cells.

Main Methods:

  • Quantitative reverse transcription polymerase chain reaction (qRT-PCR) to analyze gene expression.
  • Cell viability, migration, invasion, and apoptosis assays (MTT, transwell, flow cytometry).
  • Western blotting to assess protein expression and signaling pathways (Wnt/β-catenin, JNK).

Main Results:

  • CAT104 was highly expressed in AML cell lines (K562, HL60).
  • CAT104 knockdown suppressed cell viability, migration, and invasion while increasing apoptosis, mediated by miR-182 inhibition.
  • miR-182 promoted cell survival, migration, and invasion by upregulating ZEB1, subsequently deactivating Wnt/β-catenin and JNK pathways.

Conclusions:

  • lncRNA-CAT104 is upregulated in leukemia cells and its suppression inhibits cell survival and metastasis via miR-182 downregulation.
  • miR-182 acts as an oncogene in AML by enhancing ZEB1 expression, leading to the deactivation of Wnt/β-catenin and JNK signaling pathways.
Keywords:
Acute myeloid leukemiaCAT104JNKWnt/β-cateninZEB1miR-182

More Related Videos

Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells09:24

Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells

Published on: August 12, 2015

9.5K
Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells11:42

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Published on: April 7, 2017

9.8K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.8K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.6K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.0K
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
5.8K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
9.3K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.6K