Related Experiment Video
Updated: Dec 30, 2025

A Rapid Automated Protocol for Muscle Fiber Population Analysis in Rat Muscle Cross Sections Using Myosin Heavy Chain Immunohistochemistry
Published on: March 28, 2017
Relationship between changes in muscle fibers and CMAP in skeletal muscle with different stages of aging
Genbin Wu1, Sudan Xu2, Biao Chen1
1Department of Orthopedics, Shanghai Tenth People's Hospital Affiliated to Tongji University Shanghai, China.
Aim:
This study aimed to investigate the relationship between the changes of muscle fibers and the changesof peripheral nerve functions (CMAP) with aging.
Methods:
Lewis rats enrolled in this study. Muscle biopsy and CMAP of tibialis anterior (TA) were measured.
Results:
CMAP amplitudes increased significantly in 6 months (P<0.05). CMAP latencies of peroneal nerves found to be significantly higher in older rats (P<0.05). In 6 months these two muscle fibers went through a significant decrease (P<0.05). Type IIb and type IIx muscle fibers increased significantly in 6 months when compared with those in 1 month (P<0.05). After 6 months, the percentages of type I and type IIa muscle fibers increased as the month increased (P<0.05). The percentages of type IIb muscle fibers and type IIx muscle fibers dropped significantly as the month increased (P<0.05). Type I and type IIa muscle fibers were negatively related to CMAP amplitudes (P<0.05). Type IIb muscle fibers were positively related to CMAP amplitudes (P<0.05).
Conclusion And Discussion:
There is a shift from the fast types (Type IIx and IIb) to slow types (Type I and IIa) in muscle fibers with agingwhich is consistent with changes in CMAP amplitude of peripheral nerves.
More Related Videos
10:10Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans
Published on: February 16, 2017
12:59Improving Strength, Power, Muscle Aerobic Capacity, and Glucose Tolerance through Short-term Progressive Strength Training Among Elderly People
Published on: July 5, 2017
Related Concept Videos
Cross-bridge Cycle
Classification of Skeletal Muscle Fibers
Slow-Twitch Muscle Fibers
Slow oxidative, muscle fibers appear red due to large numbers of capillaries and high levels of...
The Effect of Aging on Tissues
Muscle Stimulation Frequency
Wave summation
At low firing rates, motor neurons induce individual twitch contractions in muscle fibers. These twitches...
Changes in the Appendicular Skeleton with Age
Initially, the limb buds consist of a core of mesenchyme covered by a layer of ectoderm. The ectoderm at the end of the limb bud thickens to form a narrow crest called the apical ectodermal ridge. This ridge stimulates the underlying...
Types of Skeletal Muscle Fibers
Fast-twitch fibers
Fast-twitch fibers, or Type II fibers, are designed for quick, powerful bursts of speed and strength. They reach peak tension within approximately 0.01 seconds following stimulation. Characterized by a large diameter and densely packed myofibrils, these fibers contain...