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miR-125b expression affects tumor growth of multiple myeloma via targeting MKK7

Yanxia Jiang1, Yajing Luan2, Dong He1

  • 1Department of Hematology, The 1st Affiliated Hospital of Nanchang University Jiangxi, China.

International Journal of Clinical and Experimental Pathology
|January 23, 2020
PubMed

Insights

MicroRNAs (miRNAs) drive tumor development. This study shows miR-125b up-regulation in multiple myeloma (MM) suppresses tumor suppressor MKK7, indicating miR-125b as a potential therapeutic target for MM.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • MicroRNAs (miRNAs) play crucial roles in cancer development.
  • The specific mechanisms by which miRNAs contribute to multiple myeloma (MM) progression are not fully understood.

Purpose of the Study:

  • To investigate the role of miR-125b in multiple myeloma (MM).
  • To determine the effect of miR-125b on mitogen-activated protein kinase 7 (MKK7) in MM.

Main Methods:

  • Quantitative real-time PCR to measure miR-125b and MKK7 expression.
  • Analysis of inverse correlation in MM patient samples and cell lines.
  • Luciferase reporter assays to confirm MKK7 as a direct target of miR-125b.
  • Assessment of cell proliferation and clonogenicity upon miR-125b manipulation.

Main Results:

  • Significant up-regulation of miR-125b and deregulation of MKK7 observed in MM.
  • Confirmed inverse correlation between miR-125b and MKK7 expression in MM.
  • miR-125b directly targets MKK7 by binding to its 3' UTR.
  • Overexpression of miR-125b decreased MKK7 expression and inhibited MM cell proliferation and clonogenicity.

Conclusions:

  • MKK7 acts as a tumor suppressor in MM, and its function is inhibited by miR-125b.
  • miR-125b neutralization of MKK7 contributes to MM progression.
  • miR-125b represents a potential novel molecular therapeutic target for MM treatment.
Keywords:
MKK7metastasismiR-125bmultiple myeloma

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