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Published on: October 27, 2020
Estrogen receptor β inhibits prostate cancer cell proliferation through downregulating TGF-β1/IGF-1 signaling
Long Xiao1, Minhui Xiao1, Min Zou1
1Department of Urology, The First People's Hospital of Yunnan Province, Kunming University of Science and Technology Kunming, Yunnan Province, P. R. China.
Abstract:
Recently, estrogen receptor β (ERβ) appears to be anti-proliferative and pro-apoptotic in normal prostate gland, but its role in androgen independent prostate cancer is limited. In this study, the expression of ERβ was overexpressed in two androgen-independent prostate cancer cell lines, PC-3 and DU145 after transfection with Ad-ERβ-EGFP virus particles. Overexpressed ERβ significantly inhibited cell proliferation in these two prostate cancer cell lines using MTT assay. Flow cytometry and Annexin V-APC/7-AAD double staining confirmed that upregulation of ERβ increased cell apoptotic rate. We found that upregulation of ERβ suppressed the expression of TGF-β1/IGF-1 expression, which could be reversed by ERβ-selective antagonist PHTPP. Consistently, TGF-β1 inhibitor LY2109761 treatment could weaken the effects of ERβ-selective antagonist PHTPP on the expression of IGF-1, survivin and bcl-2 in prostate cancer cells. In conclusion, these results suggest that estrogen may play an important role in androgen-independent prostate cancer cell proliferation through ERβ-mediated suppression of TGF-β1/IGF-1.
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