Serum miRNA expression and correlation with clinical characteristics in acute kidney injury
Yinfeng Ma1, Jun Fu1, Le Qian1
1Department of Urology, The Second Affiliated Hospital of Zhejiang Chinese Medicine University Hangzhou, Zhejiang, China.
Abstract:
Acute kidney injury (AKI) is a common clinical emergency. Its fatality rate and mortality exhibit a rising trend following the increase of aging population. MiRNA participates in disease occurrence and development via targeting mRNA expression. Previous study indicated that miRNA expression was different in peripheral blood from AKT patients. However, there is still lack of related report about miRNA expression spectrum and correlation with clinical features. MiRNA microarray was applied to test miRNA expression in the serum of acute kidney injury patients. qRT-PCR was adopted to verify the differentially expressed miRNAs. Their correlation with the staging was analyzed. A total of 14 miRNAs exhibited upregulation, while 10 miRNAs presented downregulation in AKI patients compared with healthy control. Real-time PCR revealed that 14 selected miRNAs were obviously different. Correlation analysis demonstrated that miR-210, miR-21, miR-34 were positively correlated with AKI clinical staging (r = 0.56, P < 0.05; r = 0.32, P < 0.05; r = 0.38, P < 0.05, respectively). MiR-16 showed negative correlation with clinical staging (r = -0.34, P < 0.05). Multiple miRNAs were differentially expressed in the serum of AKI patients. MiR-210, miR-21, and miR-34 were correlated with AKI staging that could be treated as the biomarker of AKI.
Insights
MicroRNAs (miRNAs) in acute kidney injury (AKI) patients
Area of Science:
- Biochemistry
- Genetics
- Nephrology
Background:
- Acute kidney injury (AKI) is a critical condition with increasing mortality, particularly in aging populations.
- MicroRNAs (miRNAs) play a role in disease development by regulating messenger RNA (mRNA) expression.
- Previous research suggests altered miRNA expression in AKI patients, but a comprehensive spectrum and clinical correlation are lacking.
Purpose of the Study:
- To investigate the miRNA expression profile in the serum of AKI patients.
- To identify specific miRNAs that correlate with the clinical staging of AKI.
Main Methods:
- Serum samples from AKI patients and healthy controls were analyzed using miRNA microarray.
- Differentially expressed miRNAs were validated using quantitative real-time PCR (qRT-PCR).
- Correlation analysis was performed between miRNA expression levels and AKI clinical staging.
Main Results:
- A total of 14 miRNAs were upregulated and 10 were downregulated in AKI patients compared to controls.
- qRT-PCR confirmed significant differences in 14 selected miRNAs.
- miR-210, miR-21, and miR-34 showed positive correlations with AKI staging (r=0.56, P<0.05; r=0.32, P<0.05; r=0.38, P<0.05).
- miR-16 exhibited a negative correlation with AKI staging (r=-0.34, P<0.05).
Conclusions:
- Multiple miRNAs are differentially expressed in the serum of individuals with AKI.
- Specific miRNAs, including miR-210, miR-21, and miR-34, are correlated with AKI clinical staging.
- These identified miRNAs hold potential as biomarkers for AKI staging.
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