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Related Experiment Videos

Corneal endothelium under various storage conditions.

R Geeraets, W D McLester, F D McMullan

    American Journal of Ophthalmology
    |February 1, 1977
    PubMed
    Summary

    Rabbit corneas stored in McCarey-Kaufman (M-K) medium showed better endothelial cell viability up to six days compared to serum or moist chambers. M-K medium is a promising option for corneal storage.

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    Area of Science:

    • Ophthalmology
    • Tissue Engineering
    • Cell Biology

    Background:

    • Corneal storage is crucial for transplantation success.
    • Evaluating different storage media is essential for preserving endothelial cell viability.

    Purpose of the Study:

    • To compare the efficacy of McCarey-Kaufman (M-K) medium, rabbit serum, and moist chamber storage for rabbit corneas.
    • To assess endothelial cell viability and corneal integrity under different storage conditions and temperatures.

    Main Methods:

    • Rabbit corneas were stored at 4°C in M-K medium, rabbit serum, and a moist chamber.
    • Corneas were also incubated at 37°C in M-K medium, serum, and Minimal Essential Medium (MEM) with additives.
    • Endothelial cell morphology, microvilli preservation, and corneal swelling were evaluated over time.

    Main Results:

    • At 4°C, M-K medium and rabbit serum maintained endothelial viability up to six days, with serum showing better microvilli preservation.
    • Moist chamber storage led to endothelial damage after 48 hours at 4°C.
    • At 37°C, serum caused rapid endothelial destruction and corneal swelling; M-K medium maintained viability up to six days, while MEM showed greater cell destruction after six days.

    Conclusions:

    • McCarey-Kaufman (M-K) medium provides superior corneal endothelial cell preservation compared to rabbit serum and moist chambers at 4°C for up to six days.
    • M-K medium is a suitable storage solution for short-term corneal preservation, maintaining endothelial integrity better than MEM at 37°C.
    • Further research into optimizing storage media and conditions is warranted to improve long-term corneal graft survival.

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