Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fractures: Bone Repair01:27

Fractures: Bone Repair

4.8K
Treatment for a fracture is based on the type of break, the bone affected, and the patient's age.
Minor fractures with no bone displacement are treated by immobilizing the fractured bone using a cast or splint. However, in the case of fractures with displaced bones, the broken bones are repositioned before immobilization to ensure successful healing without deformation and loss of function. The realignment of fractured bone ends is performed through a process called reduction. If the...
4.8K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

9.7K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
9.7K
Bone Remodeling01:40

Bone Remodeling

40.1K
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
40.1K
Hormones and Bone Tissue01:17

Hormones and Bone Tissue

3.6K
The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
3.6K
Spongy Bone01:09

Spongy Bone

7.3K
All bones comprise an outer layer of compact bone, and an interior made up of spongy bone tissue, also called cancellous or trabecular bone. In long bones, spongy bone tissue is mainly found in the interior of the epiphyses (broad ends of the bone).
Spongy bone is more porous, and less dense compared to compact bone. It is composed of concentric lamellae that are arranged irregularly to form the trabecular network. In some bones, the spaces between trabeculae contain red marrow, where...
7.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Activation of TAS2R Signaling by Diphenidol Suppresses Tumor Growth and Remodels the Tumor Immune Microenvironment in Oral Squamous Cell Carcinoma.

Cancers·2026
Same author

Matrix components in the developing bones and cartilage of embryonic chick skulls: an in situ hybridization study.

Anatomical science international·2026
Same author

Bone Compatibility of Experimental Ti-Ag and Ti-Cu Alloy Dental Implants in a Beagle Dog Model.

Journal of functional biomaterials·2026
Same author

Anatomical analysis of the elongated styloid process with reference to the styloglossus and stylopharyngeus muscles and ossified stylohyoid ligament: a cadaveric study.

Anatomical science international·2026
Same author

Genetic profile of Lepr<sup>+</sup> skeletal stem/progenitor cells in the periodontal ligament and functional compensation during aging and bone repair.

Bone·2026
Same author

Expression of collagen type III and tenascin-C during joint formation in embryonic chick skull.

Anatomical science international·2026

Related Experiment Video

Updated: Dec 30, 2025

Establishing a Diaphyseal Femur Fracture Model in Mice
05:46

Establishing a Diaphyseal Femur Fracture Model in Mice

Published on: December 9, 2022

2.8K

Sonic Hedgehog Regulates Bone Fracture Healing.

Hiroaki Takebe1, Nazmus Shalehin1, Akihiro Hosoya1

  • 1Division of Histology, Department of Oral Growth and Development, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu Hokkaido 061-0293, Japan.

International Journal of Molecular Sciences
|January 24, 2020
PubMed
Summary

Sonic hedgehog (Shh) plays a crucial role in bone fracture healing. This study shows Shh signaling is involved in both intramembranous and endochondral ossification during the healing process.

Keywords:
animal experimentfracture healingsonic hedgehogstem cell

More Related Videos

The Generation of Closed Femoral Fractures in Mice: A Model to Study Bone Healing
05:53

The Generation of Closed Femoral Fractures in Mice: A Model to Study Bone Healing

Published on: August 16, 2018

16.1K
Real-Time Imaging of CCL5-Induced Migration of Periosteal Skeletal Stem Cells in Mice
06:10

Real-Time Imaging of CCL5-Induced Migration of Periosteal Skeletal Stem Cells in Mice

Published on: September 16, 2020

2.5K

Related Experiment Videos

Last Updated: Dec 30, 2025

Establishing a Diaphyseal Femur Fracture Model in Mice
05:46

Establishing a Diaphyseal Femur Fracture Model in Mice

Published on: December 9, 2022

2.8K
The Generation of Closed Femoral Fractures in Mice: A Model to Study Bone Healing
05:53

The Generation of Closed Femoral Fractures in Mice: A Model to Study Bone Healing

Published on: August 16, 2018

16.1K
Real-Time Imaging of CCL5-Induced Migration of Periosteal Skeletal Stem Cells in Mice
06:10

Real-Time Imaging of CCL5-Induced Migration of Periosteal Skeletal Stem Cells in Mice

Published on: September 16, 2020

2.5K

Area of Science:

  • Bone biology
  • Developmental biology
  • Regenerative medicine

Background:

  • Bone fracture healing integrates intramembranous and endochondral ossification.
  • Indian hedgehog (Ihh) is known to promote chondrogenesis in fracture healing.
  • The precise role of Sonic hedgehog (Shh) in fracture healing remained unclear.

Purpose of the Study:

  • To investigate the involvement of Sonic hedgehog (Shh) in the process of bone fracture healing.
  • To elucidate the specific mechanisms by which Shh influences ossification during fracture repair.

Main Methods:

  • Fracture model created in six-week-old Sprague-Dawley rats and Gli-CreERT2; tdTomato mice.
  • Localization analysis of Shh and Gli1 during the fracture healing timeline.
  • Immunohistochemical examination of osteoblast markers (Runx2, Osx) and Gli1 progeny cells.

Main Results:

  • Shh and Gli1 were found to be localized during fracture healing.
  • Gli1 progeny cells exhibited similar localization patterns to osterix (Osx)-positive cells.
  • Shh and Gli1 co-localized with key osteoblast markers, Runx2 and Osx, on new bone matrix and chondrocytes.

Conclusions:

  • Sonic hedgehog (Shh) signaling is actively involved in bone fracture healing.
  • Shh participates in both intramembranous and endochondral ossification pathways during repair.
  • Findings highlight Shh as a potential therapeutic target for enhancing fracture healing.