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Disassembly of Chromophore-Guided DNA Duplexes through Site-Selective Binding of Coralyne to Pyrene-Modified Adenine
Ki Tae Kim1, Tae Su Choi1,2, Ka Young Kim1
1Department of Chemistry, Division of Advanced Materials Science, Pohang University of Science and Technology, Pohang, 37673, Republic of Korea.
Abstract:
Under ambient conditions the disassembly of self-duplexes of Py A-modified oligodeoxyadenylates (i.e., chromophore-guided DNA assemblies) through intercalative binding of coralyne units to pyrene-modified adenine (Py A) bases was carried out. The coralyne units, intercalated site-selectively on the 3' sides of the Py A units, are stabilized through π-π stacking interactions. This competitive intercalation destabilizes the stacking interactions of pairs of Py A units, resulting in dissociation of the self-duplexes. This behavior, which is opposite to that of coralyne-induced self-duplex formation of natural oligodeoxyadenylates, was exploited to prepare new types of DNA modules that can be switched between two different duplex states.
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