Related Experiment Video
Updated: Dec 30, 2025

Following Cell-fate in E. coli After Infection by Phage Lambda
Published on: October 14, 2011
The ea22 gene of lambdoid phages: preserved prolysogenic function despite of high sequence diversity
Aleksandra Dydecka1, Sylwia Bloch2, Agnieszka Necel1
1Department of Molecular Biology, Faculty of Biology, University of Gdańsk, Wita Stwosza 59, 80-308, Gdańsk, Poland.
Abstract:
The exo-xis region of lambdoid phages contains open reading frames and genes that appear to be evolutionarily important. However, this region has received little attention up to now. In this study, we provided evidence that ea22, the largest gene of this region, favors the lysogenic pathway over the lytic pathway in contrast to other characterized exo-xis region genes including ea8.5, orf61, orf60a, and orf63. Our assays also suggest some functional analogies between Ea22 and the phage integrase protein (Int). While it is unsurprising that Ea22 operates similarly in both λ and Stx phages, we have observed some distinctions that may arise from considerable sequence dissimilarity at the carboxy termini of each protein.
Related Concept Videos
Viral Replication: Lysogenic Cycle
DNA Bacteriophages
Lysogenic Cycle of Bacteriophages
Lytic Cycle of Bacteriophages
Viral Replication: Lytic Cycle
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon...

