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Osthole inhibited cell proliferation and induced cell apoptosis through decreasing CPEB2 expression via up-regulating

Kena Lu1, Jiajing Lin2, Jun Jiang3

  • 1Department of Gynecology, The First Affiliated Hospital of Guangxi University of Chinese Medicine, Guangxi Zhuang Autonomous Region, Nanning City, China.

Journal of Receptor and Signal Transduction Research
|January 24, 2020
PubMed

Insights

Osthole effectively inhibits endometrial carcinoma (EC) cell proliferation and promotes apoptosis by upregulating miR-424, which downregulates CPEB2 expression. This study suggests osthole as a potential therapeutic agent for EC treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Endometrial carcinoma (EC) is a significant cause of cancer-related mortality in women.
  • The poor prognosis of EC necessitates the identification of novel therapeutic targets and agents.
  • The precise mechanism of osthole's action in EC remains largely unexplored.

Purpose of the Study:

  • To investigate the mechanism underlying osthole's effects on endometrial carcinoma cells.
  • To determine if osthole influences cell proliferation, apoptosis, and cell cycle.
  • To elucidate the role of the miR-424/CPEB2 axis in osthole-mediated EC inhibition.

Main Methods:

  • Treatment of Ishikawa and KLE cells with varying doses of osthole.
  • Assessment of cell proliferation, apoptosis, and cell cycle.
  • Western blot analysis for apoptosis-related proteins.
  • Quantitative RT-PCR (qRT-PCR) for miR-424 expression.
  • Luciferase reporter assay to confirm miR-424 targeting of CPEB2.

Main Results:

  • Osthole significantly inhibited EC cell proliferation and induced apoptosis in a dose-dependent manner.
  • Osthole treatment led to increased miR-424 levels and decreased cytoplasmic polyadenylation element binding protein 2 (CPEB2) expression.
  • CPEB2 was confirmed as a direct target of miR-424, and its overexpression reversed osthole's effects.
  • Osthole's mechanism involves upregulating miR-424 to suppress CPEB2 expression.

Conclusions:

  • Osthole demonstrates anti-cancer properties against endometrial carcinoma by modulating the miR-424/CPEB2 pathway.
  • The findings highlight osthole as a potential therapeutic candidate for endometrial carcinoma.
  • Further research into osthole's clinical efficacy in EC is warranted.
Keywords:
Ostholeapoptosiscytoplasmic polyadenylation element-binding protein 2endometrial carcinomamiR-424proliferation

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